A protein secreted by the Salmonella type III secretion system controls needle filament assembly.

ELife
Junya KatoJ E Galán

Abstract

Type III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Salmonella Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded within the T3SS gene cluster. Absence of OrgC results in significantly reduced number of needle substructures but does not affect needle length. We show that OrgC is secreted by the T3SS and that exogenous addition of OrgC can complement a ∆orgC mutation. We also show that OrgC interacts with the needle filament subunit PrgI and accelerates its polymerization into filaments in vitro. The structure of OrgC shows a novel fold with a shared topology with a domain from flagellar capping proteins. These findings identify a novel component of T3SS and provide new insight into the assembly of the type III secretion machine.

References

Mar 1, 1991·Journal of Bacteriology·T Michiels, G R Cornelis
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Jan 29, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D S Wishart, A M Nip
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T KuboriJ E Galán
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sonja A DamesPeter E Wright
Oct 24, 2003·Infection and Immunity·James B Day, Catherine A Lee
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Saori Maki-YonekuraKeiichi Namba
Aug 20, 2004·Methods in Molecular Biology·Bruce A Johnson
Aug 20, 2004·Methods in Molecular Biology·Peter Güntert
Nov 6, 2004·Science·Thomas C MarlovitsVinzenz M Unger
Aug 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Janet E DeaneSusan M Lea
Sep 19, 2006·Journal of Bacteriology·Andrés AguirreFernando C Soncini
Jul 10, 2007·Journal of Molecular Biology·Yu WangRoberto N De Guzman
Jun 17, 2009·Cell Host & Microbe·Jorge E Galán
Dec 9, 2009·Molecular Microbiology·Bill BlaylockOlaf Schneewind
Jun 15, 2010·Nature Structural & Molecular Biology·Omer PoyrazMichael Kolbe
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samuel WagnerJorge E Galán
Jun 2, 2012·The Journal of Biological Chemistry·Dalian ZhongRoberto N De Guzman
Jun 16, 2012·Nature·Antoine LoquetAdam Lange
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Matthew D Lefebre, Jorge E Galán
Feb 4, 2014·FEMS Microbiology Reviews·Andreas Diepold, Samuel Wagner
Jul 9, 2014·Annual Review of Microbiology·Jorge E GalánSamuel Wagner
Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Andreas Diepold, Judith P Armitage
Mar 22, 2016·Microbiology Spectrum·Ryan Q Notti, C Erec Stebbins

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Citations

Dec 1, 2020·Molecular Microbiology·Bailey Milne-DaviesAndreas Diepold
Nov 6, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Fernanda de Oliveira BarbosaAngelo Berchieri Junior
Apr 20, 2021·Frontiers in Cellular and Infection Microbiology·Lele LianJianjun Dai
Apr 4, 2019·EcoSal Plus·Maria Lara-Tejero, Jorge E Galán
Jul 1, 2021·Microbiology and Molecular Biology Reviews : MMBR·Eduardo A GroismanJeongjoon Choi
Oct 16, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Lele LianJianjun Dai

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Datasets Mentioned

BETA
AKV07676
CAS11480
CAC79556
KIT21774
AAO18031

Methods Mentioned

BETA
electron microscopy
gel-filtration
affinity purification
two-hybrid
dynamic light scattering
NMR
X-ray
two hybrid
ion-exchange chromatography
size exchange chromatography

Software Mentioned

OrgC
BioEdit
NMRPipe
Precision Deconvolve
AMBER7
NMRView
DALI
Pymol
Muscle
CYANA

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