A protein subunit of human RNase P, Rpp14, and its interacting partner, OIP2, have 3'-->5' exoribonuclease activity

Proceedings of the National Academy of Sciences of the United States of America
Taijiao Jiang, S Altman

Abstract

The processing of precursor tRNAs at their 5' and 3' termini is a fundamental event in the biosynthesis of tRNA. RNase P is generally responsible for endonucleolytic removal of a leader sequence of precursor tRNA to generate the mature 5' terminus. However, much less is known about the RNase P counterparts or other proteins that are active at the tRNA 3' terminus. Here we show that one of the human RNase P subunits, Rpp14, together with one of its interacting protein partners, OIP2, is a 3'-->5' exoribonuclease with a phosphorolytic activity that processes the 3' terminus of precursor tRNA. Immunoprecipitates of a crude human RNase P complex can process both ends of precursor tRNA by hydrolysis, but purified RNase P has no exonuclease activity. Rpp14 and OIP2 may be part of an exosome activity.

References

Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·P S EderS Altman
Oct 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·M P Deutscher, Z Li
Jan 6, 2001·RNA·S AltmanA Vioque
Jan 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Li, S Altman
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·T Jiang, S Altman
Mar 17, 2001·EMBO Reports·M Mörl, A Marchfelder
Jun 20, 2001·The Journal of Biological Chemistry·H van EenennaamG J Pruijn

❮ Previous
Next ❯

Citations

Aug 19, 2007·Molecular BioSystems·Sidney Altman
Jan 7, 2006·Nucleic Acids Research·Geurt SchildersGer J M Pruijn
Apr 1, 2011·Nucleic Acids Research·Robert ReinerNayef Jarrous
Sep 21, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sidney Altman
Jul 16, 2010·RNA·Olga Esakova, Andrey S Krasilnikov
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elizaveta KovriginaSidney Altman
Dec 19, 2006·Cell·Quansheng LiuChristopher D Lima
Apr 6, 2012·Trends in Genetics : TIG·Sandra L WolinXinguo Chen
Oct 4, 2017·RNA·Venkat GopalanAndrey S Krasilnikov
Apr 17, 2020·Learning & Memory·Michaelina N NarteyJohn H McLean
Mar 21, 2008·The Journal of Biological Chemistry·Marcos V A S NavarroBeatriz G Guimarães
Sep 7, 2004·European Journal of Cell Biology·Reinout RaijmakersGer J M Pruijn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.