A Proteolytic Complex Targets Multiple Cell Wall Hydrolases in Pseudomonas aeruginosa

MBio
Disha SrivastavaAndrew J Darwin

Abstract

Carboxy-terminal processing proteases (CTPs) occur in all three domains of life. In bacteria, some of them have been associated with virulence. However, the precise roles of bacterial CTPs are poorly understood, and few direct proteolytic substrates have been identified. One bacterial CTP is the CtpA protease of Pseudomonas aeruginosa, which is required for type III secretion system (T3SS) function and for virulence in a mouse model of acute pneumonia. Here, we have investigated the function of CtpA in P. aeruginosa and identified some of the proteins it cleaves. We discovered that CtpA forms a complex with a previously uncharacterized protein, which we have named LbcA (lipoprotein binding partner of CtpA). LbcA is required for CtpA activity in vivo and promotes its activity in vitro We have also identified four proteolytic substrates of CtpA, all of which are uncharacterized proteins predicted to cleave the peptide cross-links within peptidoglycan. Consistent with this, a ctpA null mutant was found to have fewer peptidoglycan cross-links than the wild type and grew slowly in salt-free medium. Intriguingly, the accumulation of just one of the CtpA substrates was required for some ΔctpA mutant phenotypes, including the defective...Continue Reading

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Citations

Aug 18, 2020·FEMS Microbiology Reviews·Astra Heywood, Iain L Lamont
Jul 22, 2020·Microbial Cell Factories·Katsuya Fuchino, Per Bruheim
May 13, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jung-Ho ShinTobias Dörr
Apr 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Pavan Kumar Chodisetti, Manjula Reddy
Feb 21, 2021·Applied and Environmental Microbiology·Joseph SaoudSébastien P Faucher
Jan 21, 2021·EcoSal Plus·Shambhavi GardeManjula Reddy

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Methods Mentioned

BETA
tandem affinity purification
Tandem affinity tag purification
amidation
light microscopy
electron microscopy
PCR

Software Mentioned

MassLynx
MatLab

Related Concepts

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Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.