A proteomic analysis of arsenical drug resistance in Trypanosoma brucei

Proteomics
Aude L FoucherC Michael R Turner

Abstract

We have undertaken 2-DE and MS to identify proteins associated with arsenical drug resistance in Trypanosoma brucei. This parasite causes sleeping sickness in humans, and arsenical drug resistance is a significant potential problem. Comparative analysis of approximately 2000 spots resolved by 2-DE in the soluble proteomes of drug-sensitive and drug-resistant isogenic lines of T. brucei identified a protein spot whose absence associated with resistance to the arsenical drug, Cymelarsan. MS matched this protein to an identical pair of tandem genes Tb09.211.0120 and 0130 that encode a putative nascent polypeptide associated complex subunit. This protein also occurs as an isoform located in both resistant and sensitive lines at a similar molecular weight, but different pI. The difference between isogenic lines was confirmed by Western blot using an antibody against recombinant protein. Both genes were identical in sequence between drug-sensitive and drug-resistant lines and both were transcribed as determined by RT-PCR. We postulate that the missing protein isoform arose due to the lack of a PTM.

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Citations

Feb 22, 2012·Parasitology·Karl Burgess, Richard Burchmore
Jun 20, 2013·Expert Review of Proteomics·Philippe HolzmullerAnne Geiger
Feb 4, 2010·Expert Review of Proteomics·Philippe HolzmullerDavid Georges Biron
Feb 15, 2011·Journal of Proteomics·Anne GeigerPhilippe Holzmuller
Jan 9, 2010·Journal of Proteomics·Patricia CuervoJose B De Jesus
Aug 15, 2009·Experimental Parasitology·Shruti SharmaChinmoy S Dey
Feb 5, 2008·Molecular and Biochemical Parasitology·Didier VertommenFred R Opperdoes
Dec 21, 2007·Proteomics·Daniel J BridgesRichard J S Burchmore
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Aug 29, 2006·Molecular and Biochemical Parasitology·Alexandra LüscherPascal Mäser
Mar 9, 2018·Proteomics. Clinical Applications·Natalia Tiberti, Jean-Charles Sanchez
Jul 1, 2020·Microorganisms·Magali Van den KerkhofGuy Caljon
Jun 30, 2010·Journal of Proteome Research·Marie-Christine BrothertonMarc Ouellette

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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.