A proteomic analysis of Helicoverpa armigera adults after exposure to UV light irradiation

Journal of Insect Physiology
Jian-Yu MengChao-Liang Lei

Abstract

Ultraviolet (UV) light (blacklight), which emits UV in the range of 320-400nm, has been used worldwide in light trapping of insect pests. To gain a better understanding of the response of Helicoverpa armigera adults to UV light irradiation, we carried out a comparative proteomic analysis. Three-day-old adults were exposed to UV light for 1h. Total proteins were extracted and separated by two-dimensional gel electrophoresis. More than 1200 protein spots were reproducibly detected, including 12 that were more abundant and 21 less abundant. Mass spectrometry analysis and database searching helped us to identify 29 differentially abundant proteins. The identified proteins were categorized into several functional groups including signal transduction, RNA processing, protein processing, stress response, metabolisms, and cytoskeleton structure, etc. This study is the first analysis of differentially expressed proteins in phototactic insects under UV light irradiation conditions and gives new insights into the adaptation mechanisms responsive to UV light irradiation stress.

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Citations

Oct 14, 2014·Pesticide Biochemistry and Physiology·JianYu MengShengHua Shang
Oct 8, 2017·Environmental Science and Pollution Research International·Yan GaoQiuying Huang
Oct 18, 2018·Environmental Science and Pollution Research International·Kil-Nam KimChao-Liang Lei
Jun 30, 2019·Pest Management Science·Kil-Nam KimChao-Liang Lei
Jun 25, 2018·Environmental Science and Pollution Research International·Changyu Zhang, Jianyu Meng
Feb 12, 2017·Environmental Science and Pollution Research International·Arif AliChao-Liang Lei

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