A proteomic analysis of placental trophoblastic cells in preeclampsia-eclampsia

Cell Biochemistry and Biophysics
Kai-dong MaXiao-tian Li

Abstract

To explore the proteomic changes of placental trophoblastic cells in preeclampsia-eclampsia (PE), placental trophoblastic cells from normally pregnant women and women with hypertension during gestational period were prepared by laser capture microdissection (LCM), and proteins isolated from these cells were subjected to labeling and proteolysis with isotope-coded affinity tag reagent. A qualitative and quantitative analysis of the proteome expression of placental trophoblastic cells was made using two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS). A total of 831 proteins in placental trophoblastic cells were identified by combined use of LCM technique and 2D LC-MS/MS. The result was superior to that of conventional two-dimensional electrophoresis method. There were marked differences in 169 proteins of placental trophoblastic cells between normally pregnant women and women with PE. Of 70 (41.4 %) proteins with more than twofold differences, 31 proteins were down-regulated, and 39 were up-regulated in placental trophoblastic cells of the woman with PE. Laminin expression in placenta trophoblastic cells of women with PE was significantly down-regulated as confirmed by Western blot analysis. These findin...Continue Reading

References

Jul 1, 1992·Fertility and Sterility·T Turpeenniemi-HujanenU Puistola
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M SasakiY Yamada
Nov 8, 1996·Science·M R Emmert-BuckL A Liotta
Apr 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Korhonen, I Virtanen
Dec 31, 1997·Science·R F BonnerL A Liotta
Apr 30, 1998·Obstetrical & Gynecological Survey·E van Beck, L L Peeters
Nov 20, 1998·American Journal of Obstetrics and Gynecology·G A Dekker, B M Sibai
Mar 5, 1999·Journal of the Society for Gynecologic Investigation·V JainR E Garfield
Apr 2, 1999·Seminars in Perinatology·Y VedernikovR E Garfield
Jun 12, 1999·Molecular Carcinogenesis·S M GoldsworthyR R Maronpot
Jul 26, 2000·Obstetrics and Gynecology·A D AllaireB A Lessey
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P GygiR Aebersold
Jan 4, 2001·Human Immunology·M O'BrienP Moreau
Feb 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Korhonen, I Virtanen
Aug 15, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·F ReisterB Huppertz
Oct 5, 2001·FEBS Letters·A GrosfeldM Guerre-Millo
Oct 20, 2001·American Journal of Obstetrics and Gynecology·M J LucasF G Cunningham
Dec 4, 2001·Journal of Reproductive Immunology·Pierre Yves Robillard
May 4, 2002·Molecular and Cellular Endocrinology·Debra Goldman-Wohl, Simcha Yagel
Jun 14, 2002·Journal of Reproductive Immunology·Fabian Arechavaleta-VelascoSamuel Parry
Nov 7, 2002·Methods in Enzymology·James L Wittliff, Mark G Erlander
Feb 19, 2003·Nature Genetics·Jeffrey A RanishRuedi Aebersold
Feb 21, 2003·Journal of the Society for Gynecologic Investigation·Yang XiaRodney E Kellems
Apr 29, 2003·Journal of Proteome Research·Michael B GosheRichard D Smith
Mar 26, 2005·Brain Research. Molecular Brain Research·Jinghua JinJing Zhang
Apr 27, 2005·Journal of Alzheimer's Disease : JAD·Jing ZhangThomas J Montine
Jan 3, 2007·Gynecologic and Obstetric Investigation·Li-zhou SunYun-shan Xiao

❮ Previous
Next ❯

Citations

May 27, 2015·International Journal of Molecular Sciences·Kai P LawPhilip N Baker
Mar 13, 2016·American Journal of Obstetrics and Gynecology·Komal KediaSteven W Graves
Jun 10, 2016·The Journal of Obstetrics and Gynaecology Research·Qi LuZhenyu Zhang
Mar 30, 2017·American Journal of Obstetrics and Gynecology·Matthew GormleySusan J Fisher
Jul 13, 2018·Expert Review of Proteomics·Christian DellesStuart A Nicklin
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Min LiuRong Zhou
Oct 22, 2019·Frontiers in Physiology·Cha HanFengxia Xue

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.