A Proteomic Analysis of Sarcoptes scabiei (Acari: Sarcoptidae)

Journal of Medical Entomology
Marjorie S MorganDavid R Cool

Abstract

The pruritic skin disease scabies is caused by the burrowing of the itch mite Sarcoptes scabiei (De Geer). It is difficult to diagnose this disease because its symptoms often resemble those of other skin diseases. No reliable blood or molecular diagnostic test is available. The aim of this project was to begin to characterize the scabies proteome to identify scabies mite proteins, including those that may be useful in the development of a diagnostic test or vaccine. Various scabies mite extracts were separated by two-dimensional electrophoresis, and 844 Coomassie Blue-stained protein spots were excised, subjected to trypsin digestion, and analyzed by Matrix Assisted Laser Desorption/Ionization Time-Of-Flight/Time-Of-Flight (MALDI-TOF/TOF) mass spectrometry (MS). Tryptic fragment sequences determined by MS were searched against the recently completed S. scabiei annotated genome, leading to the identification of >150 proteins. Only 10 proteins hit to previously identified scabies proteins including actin, tropomyosin, and several ABC transporters. Thirteen proteins had homology to dust mite allergens (members of groups 8, 10, 13, 17, 20, 25, and 28). Most other sequences showed some homology to proteins in other mites and ticks i...Continue Reading

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Citations

Jul 3, 2016·Parasites & Vectors·Larry G ArlianS Dean Rider
Jun 22, 2017·Parasites & Vectors·Larry G Arlian, Marjorie S Morgan
Aug 12, 2017·Parasites & Vectors·Sajad A BhatShelley F Walton
Oct 2, 2020·PLoS Neglected Tropical Diseases·Pasi K KorhonenKatja Fischer
Aug 28, 2020·Animals : an Open Access Journal From MDPI·Marwa A AhmedMostafa F N Abushahba
Jul 16, 2019·International Journal for Parasitology. Parasites and Wildlife·Kevin D NiedringhausMichael J Yabsley
Mar 26, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Emmanuel Edwar Siddig, Roderick Hay
Mar 20, 2020·Trends in Parasitology·Galia Ramírez-TolozaArturo Ferreira
Sep 30, 2019·International Archives of Allergy and Immunology·Eduardo Santos da SilvaNeuza Maria Alcantara-Neves
Dec 4, 2020··Muhsin A. AL-DhalimiSaleem Khteer Al-Hadraawy

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