A proteomic approach to analyze salt-responsive proteins in rice leaf sheath

Proteomics
Fida Mohammad Abbasi, Setsuko Komatsu

Abstract

To examine the response of rice to salt stress, changes in protein expression were analyzed using a proteomic approach. To investigate dose- and time-dependent responses, rice seedlings were exposed to 50, 100 and 150 mM NaCl for 6 to 48 h. Proteins were extracted from leaf sheath and separated by two-dimensional polyacrylamide gel electrophoresis. Eight proteins showed 1- to 3-fold up-regulation in leaf sheath, in response to 50 mM NaCl for 24 h. Among these, three proteins were unidentified (LSY081, LSY262 and LSY363) while five proteins were identified as fructose bisphosphate aldolases, photosystem II (PSII) oxygen evolving complex protein, oxygen evolving enhancer protein 2 (OEE2) and superoxide dismutase (SOD). The maximum expression levels of seven proteins were at 24 h. Their expression declined after 48 h of 50 mM NaCl treatment. In contrast, SOD maintained its elevated expression throughout these conditions. The increased expression of proteins seen in the 50 mM NaCl treatment group was less pronounced in the groups receiving 100 or 150 mM NaCl for 24 h. The expression of SOD was a common response to cold, drought, salt and abscisic acid (ABA) stresses while the expression of LSY081, LSY363 and OEE2 was enhanced by sa...Continue Reading

Citations

Jun 15, 2011·Current Microbiology·Langyong MaoKeping Chen
Feb 12, 2008·Amino Acids·K AghaeiS Komatsu
Jul 5, 2006·Functional & Integrative Genomics·Chandan SahiAnil Grover
Dec 29, 2010·Plant Molecular Biology·Hong YaoJames A Birchler
Oct 24, 2012·Applied Biochemistry and Biotechnology·Khalid Rehman HakeemMuhammad Iqbal
Dec 7, 2010·Molecular Biotechnology·Raveendran MuthurajanJohn Bennett
Oct 25, 2011·Journal of Proteome Research·Heng ZhangShaojun Dai
Sep 28, 2013·Journal of Proteome Research·Lingxia WangWei Chen
Nov 21, 2013·Journal of Proteome Research·Cinthya Mirella PachecoTercilio Calsa
Aug 30, 2013·Journal of Proteome Research·Raksha Singh, Nam-Soo Jwa
Nov 3, 2011·Omics : a Journal of Integrative Biology·Arafet ManaaMireille Faurobert
Oct 11, 2012·Omics : a Journal of Integrative Biology·Khalid Rehman HakeemMunir Ozturk
Jan 10, 2009·Plant & Cell Physiology·Yuichi Tada, Takaaki Kashimura
Mar 31, 2010·Proteome Science·Hamid SobhanianSetsuko Komatsu
Sep 11, 2007·Bioscience, Biotechnology, and Biochemistry·Sahar Nohzadeh MalakshahGhasem Hosseini Salekdeh
Jun 4, 2014·PloS One·Aline Melro MuradBetania Ferraz Quirino
Dec 18, 2013·Analytical and Bioanalytical Chemistry·Anna Laura CapriottiAldo Laganà
Jan 21, 2014·World Journal of Microbiology & Biotechnology·Caixia ZhangShihua Shen
Jul 24, 2014·Pesticide Biochemistry and Physiology·Xinyue MeiXili Liu
Jul 28, 2013·Journal of Proteomics·Isabel A AbreuM Margarida Oliveira
Apr 9, 2013·Journal of Proteomics·Abu Hena Mostafa KamalSun Hee Woo
Feb 7, 2013·Journal of Proteomics·Qi ZhaoShaojun Dai
Jan 30, 2013·Journal of Photochemistry and Photobiology. B, Biology·Anirban GuhaAttipalli Ramachandra Reddy
Aug 8, 2012·Plant Physiology and Biochemistry : PPB·Elham SarhadiGhasem Hosseini Salekdeh
Feb 9, 2011·Biochimica Et Biophysica Acta·Heeyoun HwangSeong Hee Bhoo
Oct 19, 2010·Journal of Plant Physiology·Weihong FanShihua Shen
Jul 4, 2009·Peptides·Roya RazavizadehSetsuko Komatsu
Mar 10, 2016·Journal of Proteomics·Joaquim A G Silveira, Fabricio E L Carvalho

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.