A proteomic characterization of the plasma membrane of human epidermis by high-throughput mass spectrometry

The Journal of Investigative Dermatology
Josip BlonderJonathan C Vogel

Abstract

Membrane proteins are responsible for many critical cellular functions and identifying cell surface proteins on different keratinocyte populations by proteomic approaches would improve our understanding of their biological function. The ability to characterize membrane proteins, however, has lagged behind that of soluble proteins both in terms of throughput and protein coverage. In this study, a membrane proteomic investigation of keratinocytes using a two-dimensional liquid chromatography (LC) tandem-mass spectrometry (MS/MS) approach that relies on a buffered methanol-based solubilization, and tryptic digestion of purified plasma membrane is described. A highly enriched plasma membrane fraction was prepared from newborn foreskins using sucrose gradient centrifugation, followed by a single-tube solubilization and tryptic digestion of membrane proteins. This digestate was fractionated by strong cation-exchange chromatography and analyzed using microcapillary reversed-phase LC-MS/MS. In a set of 1306 identified proteins, 866 had a gene ontology (GO) annotation for cellular component, and 496 of these annotated proteins (57.3%) were assigned as known integral membrane proteins or membrane-associated proteins. Included in the iden...Continue Reading

References

May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 5, 1997·Nature·K Simons, E Ikonen
Oct 31, 1997·Journal of the American Academy of Dermatology·S C BaerM Younes
Jul 22, 1998·Virchows Archiv : an International Journal of Pathology·R Moll, I Moll
Apr 29, 2000·Electrophoresis·V SantoniT Rabilloud
Jan 9, 2001·Trends in Cell Biology·D P KelsellM B Hodgins
Jun 21, 2001·Archives of Dermatological Research·M HentulaS Peltonen
Sep 29, 2001·Science·R G Parton
Apr 11, 2002·Nature Cell Biology·Colin Jamora, Elaine Fuchs
Aug 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Antonius KollerJohn R Yates
Aug 23, 2002·Trends in Cell Biology·Daisuke TsurutaJonathan C R Jones
Feb 26, 2003·Cell·Mirna Perez-MorenoElaine Fuchs
Mar 1, 2003·Nature Biotechnology·Christine C Wu, John R Yates
Mar 22, 2003·The Journal of Investigative Dermatology·G N SandoK C Madison
Apr 15, 2003·Nature Biotechnology·Christine C WuJohn R Yates
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Leonard J FosterMatthias Mann

❮ Previous
Next ❯

Citations

Feb 3, 2007·Journal of Proteome Research·Xiaoting TangJames E Bruce
Jun 16, 2010·Molecular & Cellular Proteomics : MCP·Noelle M Griffin, Jan E Schnitzer
Dec 9, 2008·Antioxidants & Redox Signaling·Hemal H Patel, Paul A Insel
Jul 1, 2009·Proteome Science·Mitchell L MeadeJohn J Schlager
Jan 13, 2009·Journal of Lipid Research·Yu Zi ZhengLeonard J Foster
Nov 11, 2015·Proteomics·Rafael S LindosoAdalberto Vieyra
Feb 2, 2011·Journal of Dermatological Science·Gilles LemaîtreGilles Waksman
Feb 5, 2010·The British Journal of Dermatology·C T OngT T Phan
Oct 17, 2009·Proteomics·Stuart J Cordwell, Tine E Thingholm
Oct 19, 2006·Journal of Biomedicine & Biotechnology·Leonel RojoRicardo B Maccioni
May 31, 2011·Journal of Proteomics·Tânia SimõesDeborah Penque
Jun 27, 2012·Peptides·Michael S ParkerSteven L Parker
Oct 8, 2005·Expert Review of Proteomics·Chun-Ming HuangCraig A Elmets
Oct 8, 2005·Expert Review of Proteomics·Francesca RaimondoMarina Pitto
Mar 31, 2006·Molecular & Cellular Proteomics : MCP·Thomas J LukasTeepu Siddique
Sep 20, 2005·Molecular & Cellular Proteomics : MCP·Rie UematsuShin-Ichiro Nishimura
May 2, 2006·Molecular & Cellular Proteomics : MCP·Johanne MorelFrançoise Simon-Plas
Mar 8, 2005·Journal of Mass Spectrometry : JMS
Aug 13, 2005·Proteomics·Gilles LemaîtreGilles Waksman
Nov 3, 2006·Expert Review of Proteomics·Josip BlonderTimothy D Veenstra
Nov 10, 2010·Amino Acids·Jacek R Wiśniewski
Apr 27, 2011·Environmental Microbiology·Dominic BurgRicardo Cavicchioli
Jun 20, 2020·Expert Review of Proteomics·Jörn DengjelAlexander Nyström
Nov 17, 2010·Analytical and Bioanalytical Chemistry·Liangliang SunYukui Zhang
Jan 31, 2019·Journal of Animal Science and Biotechnology·J J BondV H Oddy
Sep 26, 2007·Expert Review of Molecular Diagnostics·Donald J Johann, Josip Blonder
Jul 28, 2020·International Journal of Molecular Sciences·Toini PemmariTero A H Järvinen
Mar 25, 2021·Journal of Cosmetic Dermatology·Jianbiao He, Yan Jia
Oct 8, 2008·Journal of Proteome Research·J M ChickM S Baker
Nov 11, 2009·Journal of Proteome Research·Leon R McQuadeMark S Baker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.