A proteomic signature that reflects pancreatic beta-cell function

PloS One
Aoife M CurranLorraine Brennan

Abstract

Proteomics has the potential to enhance early identification of beta-cell dysfunction, in conjunction with monitoring the various stages of type 2 diabetes onset. The most routine method of assessing pancreatic beta-cell function is an oral glucose tolerance test, however this method is time consuming and carries a participant burden. The objectives of this research were to identify protein signatures and pathways related to pancreatic beta-cell function in fasting blood samples. Beta-cell function measures were calculated for MECHE study participants who completed an oral glucose tolerance test and had proteomic data (n = 100). Information on 1,129 protein levels was obtained using the SOMAscan assay. Receiver operating characteristic curves were used to assess discriminatory ability of proteins of interest. Subsequent in vitro experiments were performed using the BRIN-BD11 pancreatic beta-cell line. Replication of findings were achieved in a second human cohort where possible. Twenty-two proteins measured by aptamer technology were significantly associated with beta-cell function/HOMA-IR while 17 proteins were significantly associated with the disposition index (p ≤ 0.01). Receiver operator characteristic curves determined th...Continue Reading

References

Feb 17, 1983·The New England Journal of Medicine·M FeldmanC H Li
Mar 1, 1984·Metabolism: Clinical and Experimental·R L ReidS S Yen
Mar 1, 1981·The Journal of Clinical Endocrinology and Metabolism·R L Reid, S S Yen
Nov 28, 2002·Diabetes Care·UNKNOWN Diabetes Prevention Program (DPP) Research Group
Nov 25, 2005·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Eleanor M ScottJohn B C Findlay
Jan 25, 2007·American Journal of Physiology. Endocrinology and Metabolism·Jinhui ZhangXiaoli Chen
Sep 3, 2008·Cell Metabolism·Alessandro DoriaC Ronald Kahn
Jul 4, 2009·Nature Reviews. Immunology·Sarah L Gaffen
Oct 15, 2010·Journal of Proteome Research·Michael MarisChantal Mathieu
Dec 18, 2010·Nature Reviews. Genetics·Albert-László BarabásiJoseph Loscalzo
Nov 15, 2011·Diabetes Research and Clinical Practice·David R WhitingJonathan Shaw
Nov 19, 2011·Nucleic Acids Research·Thomas KelderAlexander R Pico
Jan 4, 2013·The American Journal of Clinical Nutrition·Miriam F RyanMichael J Gibney
Feb 5, 2013·Biochimica Et Biophysica Acta·Paromita Mukherjee, Sridhar Mani
Apr 2, 2013·Frontiers in Endocrinology·Marlon E Cerf
Oct 12, 2013·The Journal of Biological Chemistry·Rong WangDouglas R Cavener
Feb 26, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A O'GormanL Brennan
Apr 3, 2014·International Journal of Proteomics·V Srinivasa RaoG N Sunand Kumar
Apr 10, 2014·Molecular BioSystems·Martina WallaceLorraine Brennan
Jul 18, 2014·PLoS Genetics·Ville-Petteri MäkinenThemistocles L Assimes
Aug 16, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Cristina MenniAna M Valdes
Jan 27, 2015·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Franscois RunauAshley Dennison
Feb 24, 2015·PLoS Computational Biology·Martina KutmonChris T Evelo
Mar 20, 2015·World Journal of Diabetes·Nidhi Bansal
Jul 8, 2015·Journal of Proteome Research·Abdelfattah El OuaamariRohit N Kulkarni
Jul 18, 2015·Expert Review of Proteomics·Yetrib Hathout
Aug 15, 2015·Journal of Diabetes Research·Samreen Riaz
Jan 7, 2016·Immunity, Inflammation and Disease·Nonantzin Beristain-CovarrubiasVianney Ortiz-Navarrete
Feb 27, 2016·The Journal of Biological Chemistry·Taiyi KuoDomenico Accili
Aug 19, 2016·PloS One·Aoife M CurranLorraine Brennan

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISAs
ELISA

Clinical Trials Mentioned

NCT01172951

Software Mentioned

WikiPathways
i2d
SOMAscan
PathVisio
SOMAmers
Biomart

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.