A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus

Frontiers in Immunology
Baozhen SunFei Zhu

Abstract

In this study, we investigated the hemocytes' immune response to white spot syndrome virus (WSSV) or Vibrio alginolyticus infection at the protein level. The differential proteomes from crab hemocytes infected with WSSV or V. alginolyticus were analyzed using the isobaric tags for relative and absolute quantitation approach immediately after infection. Using this approach, we identified 1,799 proteins by their by LC-MS/MS spectra and sequencing data. These included 157 upregulated proteins and 164 downregulated proteins after WSSV infection. Similarly, 243 proteins were determined to be differentially expressed during V. alginolyticus infection, of these, 121 were upregulated and 122 were downregulated after infection. Interestingly, among these differentially expressed proteins, 106 were up- or downregulated significantly in both WSSV and V. alginolyticus infection. Six genes, β-actin, myosin-9, anti-lipopolysaccharide factor isoform 4, anti-lipopolysaccharide factor 4, transketolase-like protein 2-like isoform 1, and sarcoplasmic calcium-binding protein 1 were chosen for further study. The expression of these genes all showed a trend of upregulation at 24 h post-WSSV or V. alginolyticus infection except for myosin-9 in respon...Continue Reading

References

Aug 27, 2002·Fish & Shellfish Immunology·Tor HaugOlaf B Styrvold
Jan 23, 2004·Diseases of Aquatic Organisms·A S Sahul HameedK Yoganandhan
Jun 30, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yongping GaoMichele G Wheatly
Jan 26, 2008·Nature Reviews. Immunology·Lynda M Stuart, R Alan Ezekowitz
May 29, 2009·Innate Immunity·Lorena VazquezEdgar Zenteno
Aug 10, 2010·Fish & Shellfish Immunology·Fang HanXiaoqing Wang
Jan 11, 2011·Journal of Invertebrate Pathology·Jean-Robert Bonami, Shuyong Zhang
Sep 17, 2013·Fish & Shellfish Immunology·Shan-Liang XuChun-Lin Wang
Dec 4, 2013·Journal of Biosciences·Vinayak BiradarDeepti Deobagkar
Jul 6, 2014·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Suparada KhanaruksombatPharima Phiriyangkul
Feb 24, 2015·Fish & Shellfish Immunology·Mingzhu HuangWei-Na Wang
Jan 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Iris Ming-Jing XuIrene Oi-Lin Ng
Dec 7, 2016·Developmental and Comparative Immunology·Thanachai MethathamKunlaya Somboonwiwat

❮ Previous
Next ❯

Citations

Jul 3, 2021·International Journal of Molecular Sciences·Charlotta EkblomIrene Söderhäll
Jul 9, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Lei LiuChunlin Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
Polymerase Chain Reaction
PCR

Software Mentioned

Mascot
SPSS Statistics
Excel
Blast 2 GO
Proteome Discoverer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.