A proteomics and transcriptomics investigation of the venom from the barychelid spider Trittame loki (brush-foot trapdoor)

Toxins
Eivind A B UndheimBryan G Fry

Abstract

Although known for their potent venom and ability to prey upon both invertebrate and vertebrate species, the Barychelidae spider family has been entirely neglected by toxinologists. In striking contrast, the sister family Theraphosidae (commonly known as tarantulas), which last shared a most recent common ancestor with Barychelidae over 200 million years ago, has received much attention, accounting for 25% of all the described spider toxins while representing only 2% of all spider species. In this study, we evaluated for the first time the venom arsenal of a barychelid spider, Trittame loki, using transcriptomic, proteomic, and bioinformatic methods. The venom was revealed to be dominated by extremely diverse inhibitor cystine knot (ICK)/knottin peptides, accounting for 42 of the 46 full-length toxin precursors recovered in the transcriptomic sequencing. In addition to documenting differential rates of evolution adopted by different ICK/knottin toxin lineages, we discovered homologues with completely novel cysteine skeletal architecture. Moreover, acetylcholinesterase and neprilysin were revealed for the first time as part of the spider-venom arsenal and CAP (CRiSP/Allergen/PR-1) were identified for the first time in mygalomorp...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·T H SzetoG F King
Feb 16, 2002·Journal of Molecular Evolution·David Posada, Keith A Crandall
Mar 26, 2003·Bioinformatics·Steve WoolleyDavid A McClellan
Apr 7, 2004·Toxicon : Official Journal of the International Society on Toxinology·Glenn F King
Oct 29, 2004·Bioinformatics·Sergei L Kosakovsky PondSpencer V Muse
Feb 4, 2005·Molecular Biology and Evolution·Ziheng YangRasmus Nielsen
Feb 11, 2005·Molecular Biology and Evolution·Sergei L Kosakovsky Pond, Simon D W Frost
Jul 5, 2006·Molecular Biology and Evolution·Sergei L Kosakovsky PondSimon D W Frost
May 8, 2007·Molecular Biology and Evolution·Ziheng Yang
May 17, 2008·International Journal of Plant Genomics·Ana Conesa, Stefan Götz
Dec 8, 2009·Biological Reviews of the Cambridge Philosophical Society·Paul A Selden, David Penney
Apr 8, 2010·Cellular and Molecular Life Sciences : CMLS·Elia Diego-GarcíaJan Tytgat
Jul 31, 2010·Bioinformatics·Wayne DelportSergei L Kosakovsky Pond
Nov 3, 2010·Nucleic Acids Research·Volker HerzigGlenn F King
Feb 22, 2011·Bioinformatics·Stefan GötzAna Conesa
Jun 15, 2011·Molecular Biology and Evolution·Sergei L Kosakovsky PondKonrad Scheffler
Nov 10, 2011·Toxins·Natalie J SaezGlenn F King
Dec 28, 2011·Systematic Biology·Norman I Platnick
Feb 10, 2012·Molecular Biology and Evolution·Kartik SunagarAgostinho Antunes
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
May 1, 2012·Toxicon : Official Journal of the International Society on Toxinology·Julie K KlintGlenn F King
Jun 29, 2012·Toxins·Monique J WindleyGraham M Nicholson
Jul 19, 2012·PLoS Genetics·Ben MurrellSergei L Kosakovsky Pond
Oct 2, 2012·Annual Review of Entomology·Glenn F King, Margaret C Hardy
Jun 12, 2013·Journal of Proteomics·Dolyce H W LowBryan G Fry

❮ Previous
Next ❯

Citations

May 13, 2014·Toxicon : Official Journal of the International Society on Toxinology·Mônica Lopes-FerreiraVidal Haddad
Nov 19, 2014·Future Medicinal Chemistry·Sandy S PinedaGlenn F King
Jul 18, 2015·Toxins·Amilcar Perez-RiverolMárcia Regina Brochetto-Braga
Sep 26, 2015·Neuro-oncology·Victor A LevinRussell C Petter
May 12, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Eivind A B UndheimGlenn F King
Jul 28, 2016·Toxins·Andreas Hougaard LaustsenMikael Engmark
Oct 1, 2016·Biologie aujourd'hui·Frédéric Ducancel
Oct 30, 2016·Toxicon : Official Journal of the International Society on Toxinology·Felipe Roberto Borba FerreiraPatrícia Maria Guedes Paiva
Mar 18, 2017·PloS One·Vera OldratiReto Stöcklin
Jan 25, 2018·Marine Drugs·Dany Domínguez-PérezAgostinho Antunes
Aug 2, 2018·PloS One·Marcelo R V DinizInácio de L Junqueira-de-Azevedo
Aug 21, 2020·Proteomics·Andrew A WalkerGlenn F King
Aug 7, 2020·Biochemical Society Transactions·Andrew A Walker
Jul 26, 2018·High-throughput·David Wilson, Norelle L Daly
Sep 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Volker HerzigBryan G Fry
Jul 1, 2019·Frontiers in Ecology and Evolution·Pamela A Zobel-ThroppGreta J Binford
Mar 25, 2019·Toxicon : Official Journal of the International Society on Toxinology·Ana L B PaivaMarcelo R V Diniz
Jul 28, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Qianqian HanZewen Liu
Aug 29, 2021·Biological Reviews of the Cambridge Philosophical Society·Tim LüddeckeAndreas Vilcinskas

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA189679

Methods Mentioned

BETA
dissection

Software Mentioned

Medical Imaging Processing , Analysis , and Visualization
Bruker ParaVision
CLC Main Workbench
HyPhy
Mixed Effects Model Evolution ( MEME )
REL
datamonkey
MEME
TreeSAAP
PAML

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Shunyi ZhuJan Tytgat
Toxicon : Official Journal of the International Society on Toxinology
Julie K KlintGlenn F King
© 2021 Meta ULC. All rights reserved