A proteomics approach to survey the antigenicity of the influenza virus by mass spectrometry

Proteomics
Bethny Morrissey, K M Downard

Abstract

A proteomics-based approach is described that combines gel electrophoresis and MS in order to identify protein interactions and the nature of the interaction interface with high-sample throughput and sensitivity. Results for protein antigens of the influenza virus have demonstrated that the approach can be successfully employed to detect determinants within the hemagglutinin antigen of two divergent type A forms of the virus in present circulation. The determinants are localised to residues 206-224 following tryptic digestion of the hemagglutinin antigen. Specific peptide-antibody complexes formed after treatment of gel-recovered antigen are shown to be able to be preserved on the MALDI target array as has been previously demonstrated in this laboratory for whole virus. The approach has broad applicability for the analysis of a wide array of protein complexes with identification of the interaction interface in a single step with high-sample throughput and at low sample levels.

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Citations

Dec 13, 2006·Journal of the American Society for Mass Spectrometry·Joshua W K HoKevin M Downard
Nov 21, 2008·Journal of the American Society for Mass Spectrometry·Ning LiuZongwei Cai
Mar 14, 2012·Analytical Chemistry·Kavya Swaminathan, Kevin M Downard
Feb 7, 2012·Journal of Agricultural and Food Chemistry·Jung-Feng Hsieh, Pei-Hsin Pan
Aug 7, 2013·Nano Letters·Christopher M GallowayRomain Quidant
Jun 27, 2007·The Analyst·Kevin M Downard, Bethny Morrissey
Jun 12, 2013·Analytical and Bioanalytical Chemistry·Kavya SwaminathanKevin M Downard
Oct 26, 2010·Journal of Virological Methods·Alexander B Schwahn, Kevin M Downard
Jun 26, 2007·Journal of Virological Methods·Bethny MorrisseyKevin M Downard
Aug 1, 2009·Proteomics. Clinical Applications·Melkamu Getie-KebtieMichail Alterman
Sep 12, 2008·Mass Spectrometry Reviews·Kevin M DownardAlexander B Schwahn
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Feb 12, 2011·Journal of Proteomics·Monika Adamczyk-PoplawskaElzbieta K Jagusztyn-Krynicka
Apr 25, 2015·Journal of Pharmaceutical and Biomedical Analysis·Patrick Müller, Kevin M Downard
Oct 26, 2010·Genomics, Proteomics & Bioinformatics·Lijun ZhangHonghao Zhou
Jul 11, 2009·Journal of Immunoassay & Immunochemistry·Alexander B Schwahn, Kevin M Downard
Jul 9, 2011·Experimental Biology and Medicine·Britta Denman, Steven R Goodman
Jan 1, 2008·European Journal of Mass Spectrometry·Alexander B SchwahnKevin M Downard
Nov 1, 2015·Biochemical Society Transactions·Vassiliy N BavroCorie Ralston
Jun 25, 2020·International Journal of Molecular Sciences·Tingting ZhaoXiuguo Hua
May 1, 2009·Analytical Chemistry·Alexander B SchwahnKevin M Downard
Sep 26, 2008·Analytical Chemistry·Bethny Morrissey, Kevin M Downard
Nov 19, 2020·ACS Infectious Diseases·Nicholas L DollmanKevin M Downard

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