A Proteomics-Based Analysis of Blood Biomarkers for the Diagnosis of COPD Acute Exacerbation.

International Journal of Chronic Obstructive Pulmonary Disease
Soo Han KimYeon-Mok Oh

Abstract

The identification of blood biomarkers to diagnose acute exacerbation of chronic obstructive pulmonary disease (AECOPD) will have clinical utility. Here, we used a proteomics-based approach to identify biomarkers capable of identifying AECOPD. This prospective, single-center pilot study enrolled 12 patients who came to Asan Medical Center (South Korea) via the outpatient clinic or emergency department with symptoms of AECOPD and were follow-up in the outpatient clinic during convalescence between 2015 and 2017. Paired blood samples collected from each patient during the treatment naïve AECOPD and convalescence stages were analyzed. A sequential window acquisition of all theoretical fragmentation spectra-mass spectrometry (SWATH-MS)-based proteome analysis was performed and a subset of the data were verified by ELISA. The SWATH-MS analysis identified 226 plasma proteins across all samples examined. The median coefficient of variation for triplicate technical replicates of each sample was 1.13 ± 1.38%, indicating high precision of the technique. Fold-change and paired t-test analyses revealed that 14 proteins were present at higher levels in the AECOPD samples than in the convalescence samples. A gene ontology analysis revealed t...Continue Reading

References

Feb 1, 1987·Annals of Internal Medicine·N R AnthonisenN A Nelson
Jun 6, 2000·The Journal of Infectious Diseases·W KhovidhunkitC Grunfeld
Jun 10, 2000·Chest·R Rodriguez-Roisin
Aug 6, 2005·European Journal of Epidemiology·Eoin MurtaghJoe MacMahon
Nov 17, 2006·The European Respiratory Journal·W R PereraJ A Wedzicha
May 18, 2007·American Journal of Respiratory and Critical Care Medicine·Klaus F RabeUNKNOWN Global Initiative for Chronic Obstructive Lung Disease
Jun 2, 2007·The European Respiratory Journal·B R Celli, P J Barnes
Sep 4, 2007·Lancet·Jadwiga A Wedzicha, Terence A R Seemungal
Nov 6, 2007·Biochimica Et Biophysica Acta·Johannes H M LevelsSander J H van Deventer
Jan 17, 2008·Chest·Karin H GroenewegenUNKNOWN COSMIC Study Group
Nov 18, 2008·The European Respiratory Journal·V RegueiroJ A Bengoechea
Apr 15, 2010·Journal of Proteome Research·Hong ChenXiangdong Wang
Feb 9, 2012·Lancet·Marc DecramerMarc Miravitlles
Apr 12, 2013·Expert Review of Respiratory Medicine·Juan Antonio Trigueros Carrero
Feb 3, 2016·Chest·Cynthia W BaffiFernando Holguin
Oct 25, 2016·Cancer Letters·Cecilia Mouronte-RoibásAlberto Ruano-Ravina
Jul 8, 2017·International Journal of Chronic Obstructive Pulmonary Disease·Wenjing RuanKejing Ying
Sep 21, 2017·Mutation Research. Reviews in Mutation Research·Younis Mohammad HazariKhalid Majid Fazili
Aug 3, 2018·European Respiratory Review : an Official Journal of the European Respiratory Society·Venkataramana K SidhayeFernando J Martinez
Aug 3, 2018·Clinical Proteomics·Antti JylhäHannu Uusitalo
Aug 15, 2018·Molecular Systems Biology·Christina LudwigRuedi Aebersold
Aug 17, 2018·Journal of Proteome Research·Milkessa HaileMariamYanbao Yu
Sep 22, 2018·The European Respiratory Journal·Victor Kim, Shawn D Aaron
Mar 16, 2019·American Journal of Respiratory Cell and Molecular Biology·Elizabeth A ReganRussell P Bowler
Mar 19, 2019·The European Respiratory Journal·H J PrinsW G Boersma
Apr 14, 2019·Methods in Molecular Biology·Qifeng LinMaxey C M Chung
Jun 14, 2019·International Journal of Chronic Obstructive Pulmonary Disease·Haipin ChenZhihua Chen
Jun 22, 2019·Journal of Cellular Physiology·Monireh MohsenzadeganRaheleh Roudi
Jul 30, 2019·PeerJ·Michael T ZimmermannGregory A Poland
Dec 19, 2019·International Journal of Chronic Obstructive Pulmonary Disease·Nozomu TakeiMasaharu Nishimura
Mar 10, 2020·World Journal of Gastroenterology : WJG·Han-Zhang ZhuChang-Ku Jia
Aug 17, 2020·Genomics, Proteomics & Bioinformatics·Tiansheng ZhuTiannan Guo

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