A protocol for chemical mutagenesis in Strongyloides ratti

Experimental Parasitology
Li GuoAdrian Streit

Abstract

Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.

References

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Nov 6, 2009·Molecular and Biochemical Parasitology·Linda NemetschkeAdrian Streit
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Dec 14, 2011·PloS One·Hannah S Seidel, Judith Kimble

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Citations

Mar 24, 2016·Parasitology·Adrian Streit
Oct 11, 2017·PLoS Pathogens·Spencer S GangElissa A Hallem
Mar 30, 2019·PLoS Pathogens·Alex Dulovic, Adrian Streit
Oct 12, 2019·Parasitology·Navonil Banerjee, Elissa A Hallem
Feb 9, 2020·The Journal of Experimental Biology·Michelle L CastellettoElissa A Hallem

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