PMID: 3770441Jun 1, 1986Paper

A protocol for estimation of cortisol plasma clearance in acid-exposed rainbow trout (Salmo gairdneri)

General and Comparative Endocrinology
S BrownT J Hara

Abstract

The cortisol metabolic clearance rate (MCR) and degradation rate (DR) were determined in acid (H2SO4)-stressed and control rainbow trout (260 g) cannulated via the dorsal aorta. Recovery from catheterization, as judged by plasma cortisol, glucose, protein, and packed cell volume (PCV), was complete by 6 days. However, serial blood sampling increased plasma cortisol. Furthermore, although no major or consistent diel change in plasma cortisol occurred in terminally sampled free-swimming control trout, fluctuations were observed in serially bled catheterized trout. These findings preclude cortisol MCR estimation by any serial sampling method. Although plasma cortisol was temporarily elevated by constant infusion (70 microliter hr-1) of saline:ethanol vehicle, a satisfactory protocol was established for determining cortisol MCR by infusion of labeled cortisol to constant plasma specific activity. The MCR for control trout in water, pH 7.7, for 7 days was 30.3 +/- 4.2 ml hr-1 100 g-1, which did not differ from that of trout in water, pH 5.0. However, the cortisol DR was greater at pH 5.0 (2.13 +/- 0.46 micrograms hr-1 100 g-1) than at pH 7.7 (0.56 +/- 0.12) due primarily to increased plasma cortisol at pH 5.0.

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Citations

Jan 1, 1994·Fish Physiology and Biochemistry·P M MazikG R Stehly
Jul 1, 1993·Fish Physiology and Biochemistry·S F Perry, S D Reid
Apr 1, 1993·Fish Physiology and Biochemistry·R G HanssenS E Bonga
Nov 2, 2004·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·M D McDonaldP J Walsh
Dec 8, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·K M GilmourS F Perry
Jul 12, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tamara M RodelaPatrick J Walsh
Aug 13, 1999·The American Journal of Physiology·C M WoodP J Walsh
Apr 29, 2011·The Journal of Experimental Biology·Sacha LeBlancSuzanne Currie
Dec 23, 2011·The Journal of Experimental Biology·Tamara M RodelaKathleen M Gilmour
Jul 19, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Chia-Hao LinPung-Pung Hwang

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