A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

MethodsX
Alisha HouseYana Sandlers

Abstract

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.

References

May 29, 2002·Cardiovascular Research·José Marín-García, Michael J Goldenthal
Dec 25, 2004·Journal of Experimental Botany·John M HalketPeter M Bramley
Aug 17, 2012·Journal of Cardiovascular Translational Research·Maxime MioulaneSian E Harding
Sep 14, 2012·Journal of Biomolecular Screening·Oksana SirenkoEvan F Cromwell
Jan 23, 2016·Journal of the American College of Cardiology·Tariq AhmadWilliam E Kraus

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Methods Mentioned

BETA
imaging techniques
amino
protein assay

Software Mentioned

SCIEX
ABSCIEX
AMDIS
Chemoview

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