A Protocol for the Isolation, Culture, and Cryopreservation of Umbilical Cord-Derived Canine Mesenchymal Stromal Cells: Role of Cell Attachment in Long-Term Maintenance.

Stem Cells and Development
Adrienne WrightMark L Weiss

Abstract

Mesenchymal stromal cells (MSCs) hold great promise in the field of regenerative medicine due to their ability to create a variable localized anti-inflammatory effect in injuries such as Crohn's disease and osteoarthritis or by incorporation in tissue engineered constructs. Currently, the MSC literature uses rodents for preclinical disease models. There is growing interest in using naturally occurring disease in large animals for modeling human disease. By review of the canine MSCs literature, it appears that canine MSCs can be difficult to maintain in culture for extended passages and this greatly varies between tissue sources, compared with human and rodent MSCs, and limited lifespan is an obstacle for preclinical investigation and therapeutic use. Research using canine MSCs has been focused on cells derived from bone marrow or adipose tissue, and the differences in manufacturing MSCs between laboratories are problematic due to lack of standardization. To address these issues, here, a stepwise process was used to optimize canine MSCs isolation, expansion, and cryopreservation utilizing canine umbilical cord-derived MSCs. The culture protocol utilizes coating of tissue culture surfaces that increases cellular adherence, increa...Continue Reading

References

Feb 8, 2006·Reproductive Biology and Endocrinology : RB&E·Ryan CarlinDeryl Troyer
Jan 24, 2007·Stem Cell Reviews·Mark L Weiss, Deryl L Troyer
Dec 11, 2007·Stem Cells·Deryl L Troyer, Mark L Weiss
Apr 30, 2008·Methods in Cell Biology·Kiran SeshareddyMark L Weiss
Aug 9, 2008·Veterinary Research Communications·F CremonesiP Mariani
Aug 19, 2009·Journal of Veterinary Science·Min-Soo SeoKyung-Sun Kang
Oct 2, 2009·Journal of Periodontology·Amber Y Spencer, Thomas E Lallier
Mar 30, 2010·Reproduction in Domestic Animals = Zuchthygiene·M F RaoufiA Barin
Sep 8, 2010·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Marco MielcarekBeverly Torok-Storb
Jun 28, 2011·Current Opinion in Immunology·Günter Lepperdinger
Dec 20, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Joshua A WoodChristopher J Murphy
Jan 24, 2012·Stem Cell Research·Eckhard U AltReza Izadpanah
Jun 27, 2012·Cell Transplantation·Min-Soo SeoKyung-Sun Kang
Aug 15, 2012·Stem Cells and Development·James HongMark L Weiss
Sep 4, 2012·BMC Veterinary Research·Hiroshi TakemitsuToshiro Arai
Dec 18, 2012·In Vitro Cellular & Developmental Biology. Animal·Seon-A ChoiMin Kyu Kim
Jul 3, 2013·Tissue Engineering. Part C, Methods·Bita CarrionAndrew J Putnam
Jan 11, 2014·The Veterinary Quarterly·Evelien de BakkerEvelyne Meyer
Aug 12, 2014·Cytotherapy·Michael BadowskiDavid T Harris
Sep 12, 2015·Transfusion·Raffaella FazzinaGiuseppina Bonanno
Mar 12, 2016·Veterinary Immunology and Immunopathology·Nathalie SaulnierStéphane Maddens
Jul 7, 2016·Stem Cells International·Mark L WeissPeter Czermak
May 17, 2017·Current Protocols in Stem Cell Biology·J Robert SmithMark L Weiss
Jun 15, 2017·Stem Cells International·Cláudia Cardoso Maciel EscalhãoTatiana Coelho-Sampaio

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Citations

Aug 19, 2020·Cells·Francesca BalzanoMargherita Maioli

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Methods Mentioned

BETA
cesarean-section
Flow Cytometry
Illumina sequencing
cesarian section
PCR

Software Mentioned

GentleMACS Dissociator
Sigma Plot
SigmaPlot
FACSDiva
ACD Systems of America s Canvas

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