PMID: 9550262Apr 29, 1998Paper

A proximal element within the human alpha 2(I) collagen (COL1A2) promoter, distinct from the tumor necrosis factor-alpha response element, mediates transcriptional repression by interferon-gamma

Matrix Biology : Journal of the International Society for Matrix Biology
K HigashiA Mauviel

Abstract

Previous studies have shown that interferon-gamma (IFN-gamma) inhibits type I collagen gene expression through both transcriptional and post-transcriptional mechanisms (Kahäri et al., 1990). In the present study, using transient cell transfections of human dermal fibroblast cultures with a series of 5' deletion promoter/CAT reporter gene constructs, we have identified the IFN-gamma-response element of the human alpha 2(I) collagen gene (COL1A2) promoter. Specifically, we have identified a segment of the proximal promoter region, located between nucleotides -161 and -125 relative to the transcription start site, as critical for down-regulation of COL1A2 promoter activity by IFN-gamma. This IFN-gamma response element (IgRE) is clearly distinct from the previously described tumor necrosis factor-alpha response element (TaRE) located between nucleotides -265 and -241 of the COL1A2 promoter, a difference which is likely to explain the additive inhibitory effect of these two cytokines. The inhibitory effect of IFN-gamma was dose-dependent and rapidly induced, requiring less than 5 min exposure of fibroblast cultures. Gel mobility shift assays indicated that a highly specific nuclear protein complex bound to this 37-base pair region o...Continue Reading

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Citations

Sep 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yutaka InagakiIsao Okazaki
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·I A EliseevaD N Lyabin
Jan 24, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stéphane Lajoie-KadochQutayba Hamid
Aug 22, 2001·The Journal of Biological Chemistry·F B RiquetM B Goldring
May 3, 2002·The Journal of Biological Chemistry·Pritam K SenguptaBarbara D Smith
Aug 15, 2003·The Journal of Biological Chemistry·Kiyoshi HigashiIwao Nakatsuka
Sep 12, 2003·The Journal of Biological Chemistry·Yong XuBarbara D Smith
Aug 16, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Georg E SteinerMichael Marberger

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