A purification method for apolipoprotein A-I and A-II

Analytical Biochemistry
M C PeitschH J Heiniger

Abstract

Apolipoproteins A-I and A-II were isolated from precipitates obtained by cold ethanol fractionation of human plasma. The starting material used in this report was precipitate B of the Kistler and Nitschmann method which corresponds approximately to fraction III of the Cohn and Oncley procedure. Through the use of urea, chloroform, and ethanol in appropriate concentrations, apolipoproteins A-I and A-II were isolated by a simple extraction technique avoiding time-consuming ultracentrifugation. Starting from 10 g of centrifuged precipitate B, approximately 100 mg of apolipoprotein A-I and 10 mg of apolipoprotein A-II were obtained. When incubated with normal human or rabbit plasma, both apolipoproteins were readily incorporated into high-density lipoproteins. Apolipoprotein A-I obtained by the cold ethanol method activated lecithin-cholesterol acyltransferase to the same extent as apolipoprotein A-I prepared by the classical flotation method. Apolipoprotein A-II had no such properties by itself, but was capable of potentiating lecithin-cholesterol acyltransferase activity of apolipoprotein A-I.

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Citations

Jan 27, 1999·Atherosclerosis·C R SirtoriG Franceschini
Apr 3, 2007·Transfusion Medicine Reviews·Thierry Burnouf
Nov 28, 2017·Bulletin of Experimental Biology and Medicine·I F UsyninA Yu Gorodetskaya
Sep 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·M N NanjeeN E Miller
Nov 5, 2013·Current Opinion in Lipidology·Brian R Krause, Alan T Remaley
Feb 9, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Junji YamashitaToshinori Nakayama

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