A purified recombinant baculovirus expressed capsid protein of a new astrovirus provides partial protection to runting-stunting syndrome in chickens.

Vaccine
Holly S SellersE Mundt

Abstract

A new viral sequence likely belonging to a virus of the family Astroviridae was determined using the gut content of chickens affected with the runting-stunting syndrome (RSS) in chickens. Since the appropriate virus could not be isolated in cell culture the open reading frame of the viral capsid protein was cloned to generate a recombinant baculovirus. The protein was purified and used as an experimental vaccine in broiler breeders to provide maternal derived antibodies for the protection of the offspring. The presence of specific antibodies was monitored by an ELISA. The offspring of vaccinated breeder hens were partially protected in a RSS challenge model.

References

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Citations

May 16, 2014·PloS One·Victoria A MeliopoulosStacey Schultz-Cherry
Aug 24, 2013·Veterinary Medicine International·Muhammad Fiaz QamarNusrat Jahan
Nov 29, 2013·Avian Diseases·J Michael Day, Laszlo Zsak
Aug 1, 2012·Avian Pathology : Journal of the W.V.P.A·Kyung-Il KangEgbert Mundt
Apr 21, 2012·Avian Pathology : Journal of the W.V.P.A·V J SmythM D Welsh
Nov 12, 2010·Archives of Virology·M J Pantin-JackwoodE Spackman
Aug 20, 2015·Avian Pathology : Journal of the W.V.P.A·A SkibinskaD Todd
Jun 17, 2016·Avian Diseases·Rüdiger HauckH L Shivaprasad
Feb 21, 2018·The Journal of General Virology·Kyung-Il KangHolly S Sellers
Jun 27, 2018·Avian Diseases·Kathleen E LongDavor Ojkić
Aug 7, 2019·Vaccines·Mehdi R M BidokhtiClaudia Baule
Jun 3, 2021·Viruses·Matthew Ykema, Yizhi J Tao

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Datasets Mentioned

BETA
AAV37186
ABX46565

Methods Mentioned

BETA
electron microscopy
PCR
electrophoresis
ELISA

Software Mentioned

Runner
ClustalW
Gene
blast
blastp

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