A purine-related metabolite negatively regulates fixNOQP expression in Sinorhizobium meliloti by modulation of fixK expression

Molecular Plant-microbe Interactions : MPMI
M SoberónJ Miranda

Abstract

5-aminoimidazole-4-carboxamide nucleotide (AICAR) is a negative effector of cytochrome terminal oxidase cbb3 production in Rhizobium etli. In this work, the effect of AICAriboside (AICAr), the precursor of AICAR on the expression of the Sinorhizobium meliloti fixNOQP operon encoding the symbiotic terminal oxidase cbb3, was analyzed. AICAr reduced the microaerobic induction levels of fixN-lacZ and fixT-lacZ gene fusions 18- and seven-fold respectively, and both genes were activated by the transcriptional activator FixK. A fixK-lacZ fusion presented 14-fold-reduced induction levels in microaerobic cell cultures in the presence of AICAr. AICAr also reduced three-fold the microaerobic expression levels of the nifA-lacZ fusion, whose expression as well as that of fixK is controlled by the two-component system FixL-FixJ. In contrast, AICAr had no effect on the expression levels of a hemA-lacZ fusion. These data suggest that AICAr prevents fixNOQP induction by the inhibition of fixK transcription.

References

Jul 1, 1987·Journal of Bacteriology·G DittaC H Kim
Jan 1, 1994·Antonie van Leeuwenhoek·J Batut, P Boistard
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P G AgronD R Helinski
Nov 5, 1999·The Journal of Biological Chemistry·A M GarneroneJ Batut

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Citations

Jun 5, 2010·Molecular Genetics and Genomics : MGG·Luzia ReutimannHauke Hennecke
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sean CrossonLucy Shapiro
Jul 29, 2021·Microbial Biotechnology·Laura A Basile, Viviana C Lepek

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