A Purkinje cell protein-2 intronic thyroid hormone response element binds developmentally regulated thyroid hormone receptor-nuclear protein complexes

Journal of Molecular Neuroscience : MN
S G HagenJ H Oppenheimer

Abstract

Two thyroid hormone response elements (TREs), designated A1 TRE (-295/-268) and B1 TRE (+207/+227), have been identified within the Purkinje cell-expressed Pcp-2 gene. Previous studies have characterized the A1 TRE (Zou et al., 1994). This article analyzes the structural and functional characteristics of the intronic B1 TRE. The B1 sequence contains four overlapping TRE half-sites. The 3' DR4 motif, consisting of the second and forth half-sites, is responsible for the T3 induction observed with the B1 sequence. Gel-shift analysis reveals developmentally regulated complexes that are abundant in the fetus and at birth and then fall precipitously in the neonate bind to B1. The observed time-course of these complexes varies inversely with the rise in Pcp-2 expression, thus raising the possibility that the complexes may represent inhibitory factors. Supershift analysis indicates that endogenous TR alpha 1 is present in the fetal nuclear protein complexes that bind to B1. Competition analysis also indicates the second B1 TRE half-site is important in binding the TR alpha 1-TRAP complexes. These studies suggest that the B1 sequence may bind potential TR alpha 1-TRAP repressor complexes in the fetus, whereas in the neonate, these TRE s...Continue Reading

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Citations

Apr 18, 2008·The Cerebellum·Grant W Anderson
Sep 21, 2002·Molecular and Cellular Endocrinology·Françoise Chantoux, Jacques Francon
Jul 4, 1997·Molecular and Cellular Endocrinology·G W AndersonJ H Oppenheimer
Dec 4, 2003·Thyroid : Official Journal of the American Thyroid Association·Grant W AndersonSidney A Jones
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thomas WeberMarlies Knipper
Mar 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Linda C HallNan Liu
Jul 3, 2002·Genome Biology·Matthew A ZapalaDavid J Lockhart
Aug 23, 2005·Vitamins and Hormones·Juan Bernal

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