A Putative Acetylation System in Vibrio cholerae Modulates Virulence in Arthropod Hosts

Applied and Environmental Microbiology
Kalle LiimattaAlexandra E Purdy

Abstract

Acetylation is a broadly conserved mechanism of covalently modifying the proteome to precisely control protein activity. In bacteria, central metabolic enzymes and regulatory proteins, including those involved in virulence, can be targeted for acetylation. In this study, we directly link a putative acetylation system to metabolite-dependent virulence in the pathogen Vibrio cholerae We demonstrate that the cobB and yfiQ genes, which encode homologs of a deacetylase and an acetyltransferase, respectively, modulate V. cholerae metabolism of acetate, a bacterially derived short-chain fatty acid with important physiological roles in a diversity of host organisms. In Drosophila melanogaster, a model arthropod host for V. cholerae infection, the pathogen consumes acetate within the gastrointestinal tract, which contributes to fly mortality. We show that deletion of cobB impairs growth on acetate minimal medium, delays the consumption of acetate from rich medium, and reduces virulence of V. cholerae toward Drosophila These impacts can be reversed by complementing cobB or by introducing a deletion of yfiQ into the ΔcobB background. We further show that cobB controls the accumulation of triglycerides in the Drosophila midgut, which sugge...Continue Reading

References

Nov 5, 1999·Journal of Molecular Biology·S Busby, R H Ebright
Jul 9, 2004·Journal of Molecular Biology·Vincent J Starai, Jorge C Escalante-Semerena
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Alan J Wolfe
Oct 5, 2005·PLoS Pathogens·Nathan S BlowPaula I Watnick
Jun 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bjoern SchwerEric Verdin
Jun 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·William C HallowsJohn M Denu
May 20, 2008·Journal of Bacteriology·Sarah V StuderEdward G Ruby
Nov 11, 2011·Journal of Computational Chemistry·Hirofumi FujimotoKozo Tsuchida
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexandra E Purdy, Paula I Watnick
Mar 15, 2012·The Journal of Biological Chemistry·Heidi A CrosbyJorge C Escalante-Semerena
Jul 9, 2013·Molecular Cell·Brian T WeinertChunaram Choudhary
Jul 16, 2013·Journal of Bacteriology·Linda I HuAlan J Wolfe
Jun 12, 2014·The Journal of Biological Chemistry·Josue BaezaJohn M Denu
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Longxiang XieJianping Xie
Dec 18, 2014·Molecular Systems Biology·Sara Castaño-CerezoManuel Cánovas
Jun 14, 2015·Applied and Environmental Microbiology·Jie RenYu-Feng Yao
Jan 27, 2016·The Journal of Infectious Diseases·Yu SangYu-Feng Yao
Jun 7, 2016·Molecular Cell·Angela M ChevertonSophie Helaine
Jul 15, 2016·Cell Metabolism·Filipe De VadderGilles Mithieux

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Citations

Sep 19, 2018·Journal of Bacteriology·Itai MuzhingiAlexandra E Purdy
May 16, 2019·Annual Review of Microbiology·Chelsey M VanDrisse, Jorge C Escalante-Semerena
Feb 4, 2021·MBio·Heidi A ButzShelley M Payne
Jul 13, 2021·Journal of Bacteriology·Aiswarya Dash, Rahul Modak
Nov 23, 2021·Frontiers in Microbiology·Ayesha NisarMingxiong He

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