A putative helical domain in the MalK subunit of the ATP-binding-cassette transport system for maltose of Salmonella typhimurium (MalFGK2) is crucial for interaction with MalF and MalG. A study using the LacK protein of Agrobacterium radiobacter as a tool

Molecular Microbiology
S WilkenE Schneider

Abstract

The ATP-binding-cassette (ABC) protein LacK of Agrobacterium radiobacter displays high sequence similarity to the MalK subunit of the Salmonella typhimurium maltose-transport system (MalFGK2). We have used LacK as a tool to identify sites of interaction of MalK with the membrane-integral components MalF and MalG. Small amounts of LacK, resulting from the expression of the plasmid-borne lacK gene, proved to be sufficient for partial restoration of growth of a malK strain of S. typhimurium on maltose. LacK failed to substitute for MalK in regulating the expression of maltose-inducible genes but the hybrid complex MalFGLacK2 was sensitive to inducer exclusion. The lacK gene also complemented a ugpC mutant of Escherichia coli to growth on sn-glycerol-3-phosphate as the phosphate source. Partially purified LacK exhibited a spontaneous ATPase activity comparable to that of MalK. A MalK"-'LacK chimeric protein was isolated (by in vivo recombination) in which the N-terminal 140 amino acids of MalK are fused to residues 141-363 of LacK. The protein substituted for MalK in maltose transport considerably better than LacK. Furthermore, random mutagenesis of the plasmid-borne lacK gene yielded three clones that were superior to wild-type la...Continue Reading

Citations

Mar 14, 2000·FEMS Microbiology Letters·A Schlösser
Dec 28, 2004·Nature Structural & Molecular Biology·Patrick H ThibodeauPhilip J Thomas
Nov 18, 2000·Infection and Immunity·H H KhunB C Lee
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·P Q Liu, G F Ames
Nov 19, 2013·PLoS Genetics·Rudra P SahaRasika M Harshey
Sep 2, 1998·Molecular Microbiology·M EhrmannA Schlösser
Dec 28, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·J Pérard, Sandrine Ollagnier de Choudens
Dec 3, 1999·Biochimica Et Biophysica Acta·J Young, I B Holland
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·W Boos, H Shuman
Mar 1, 1997·Journal of Bacteriology·A SchlösserH Schrempf
Sep 1, 2010·EcoSal Plus·Amy L Davidson, Frances Joan D Alvarez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.