Abstract
A gene, mgt, encoding a protein homologous to the N-terminal module of class A high-molecular-mass penicillin-binding proteins was identified in Ralstonia eutropha. By using specific antibodies, the corresponding Mgt protein was detected in association with the membrane, confirming that the N-terminal hydrophobic segment functioned as a membrane anchor. A derivative in which the hydrophobic sequence was deleted was overexpressed as a maltose-binding fusion protein in Escherichia coli. Cleavage of the product resulted in substantial amounts of soluble Mgt derivative, indicating that folding occurs independently on other proteins or on homologous domains of penicillin-binding proteins.
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1978·FEBS Letters·Y Van HeijenoortJ Van Heijenoort
Jul 1, 1992·Journal of Bacteriology·C MartinR Hakenbeck
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 15, 1988·Gene·C di GuanH Inouye
Dec 30, 1988·Gene·C V MainaC D Guan
Oct 1, 1986·Antimicrobial Agents and Chemotherapy·R HakenbeckA Tomasz
Jan 1, 1984·Analytical Biochemistry·M S BlakeE C Gotschlich
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Feb 1, 1984·Journal of Bacteriology·W Park, M Matsuhashi
Feb 11, 1980·FEBS Letters·Y van Heijenoort, J van Heijenoort
Feb 11, 1980·FEBS Letters·H SuzukiJ van Heijenoort
Oct 1, 1981·Journal of Bacteriology·S GottesmanP Trisler
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Sep 1, 1993·FEMS Microbiology Letters·D JendrossekA Steinbüchel
Mar 1, 1996·Nature Structural Biology·S ParesO Dideberg
May 15, 1996·The Biochemical Journal·C C WangR A Nicholas
Jan 1, 1995·Microbiology and Immunology·E YabuuchiY Nishiuchi
Aug 26, 1996·FEBS Letters·M Di BerardinoM Gubler
Feb 1, 1996·Molecular Microbiology·B G SprattM J Merrick
Citations
Apr 26, 2001·Glycobiology·J van Heijenoort
Nov 5, 1999·The Journal of Biological Chemistry·G Schiffer, J V Höltje
Nov 26, 2010·Critical Reviews in Biotechnology·Ankur GautamRupinder Tewari
Oct 29, 1998·Journal of Bacteriology·A M Di GuilmiT Vernet
Jul 24, 2001·Journal of Bacteriology·Q M WangP L Skatrud
Feb 20, 2003·Journal of Bacteriology·Anne Marie Di GuilmiThierry Vernet
Sep 24, 2004·Biochemistry·Dianah S BarrettSuzanne Walker
Dec 4, 2001·Chemistry & Biology·R Sinha RoyK Chapman
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·C Goffin, J M Ghuysen
Nov 22, 2005·Biochemical Pharmacology·Judy HallidayWim Meutermans