A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber (Cucumis sativus L.).

Journal of Experimental Botany
Zheng LiRun Cai

Abstract

It is well established that the plant hormone ethylene plays a key role in cucumber sex determination. Since the unisexual control gene M was cloned and shown to encode an ethylene synthase, instead of an ethylene receptor, the 'one-hormone hypothesis', which was used to explain the cucumber sex phenotype, has been challenged. Here, the physiological function of CsACS2 (the gene encoded by the M locus) was studied using the transgenic tobacco system. The results indicated that overexpression of CsACS2 increased ethylene production in the tobacco plant, and the native cucumber promoter had no activity in transgenic tobacco (PM). However, when PM plants were treated with exogenous ethylene, CsACS2 expression could be detected. In cucumber, ethylene treatment could also induce transcription of CsACS2, while inhibition of ethylene action reduced the expression level. These findings suggest a positive feedback regulation mechanism for CsACS2, and a modified 'one-hormone hypothesis' for sex determination in cucumber is proposed.

References

Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·H ItzhakiW R Woodson
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J MontgomeryR L Fischer
Dec 10, 1998·Nucleic Acids Research·K HigoT Korenaga
Jun 28, 2001·Plant & Cell Physiology·S YamasakiH Takahashi
Apr 16, 2004·The Plant Cell·Milos Tanurdzic, Jo Ann Banks
Oct 19, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H Mibus, T Tatlioglu
Feb 24, 2006·Vitamins and Hormones·Seiji YamasakiHideyuki Takahashi
Jul 17, 2007·Journal of Experimental Botany·Sayoko SaitoHideyuki Takahashi
Aug 21, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zheng LiRun Cai
Dec 25, 2009·The Plant Journal : for Cell and Molecular Biology·Dong-Hui WangShu-Nong Bai

❮ Previous
Next ❯

Citations

May 3, 2014·Journal of Experimental Botany·Yan ZhangHuazhong Ren
Dec 3, 2014·Plant Physiology and Biochemistry : PPB·Susana ManzanoManuel Jamilena
May 16, 2014·International Journal of Molecular Sciences·Huai-Juan XiaoZhen-Hui Gong
Mar 1, 2016·Frontiers in Plant Science·Rómulo SobralMaria M R Costa
Mar 5, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hanqiang LiuZhihui Cheng
Sep 10, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junyi TanZheng Li
Apr 1, 2014·Current Opinion in Plant Biology·Jisen ZhangRay Ming
Jul 13, 2016·Molecular Plant·Huiming ChenSanwen Huang
Dec 29, 2016·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Naoya UrasakiHideo Matsumura
May 19, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shanshan HouZheng Li
Sep 12, 2018·Journal of Experimental Botany·Huanhuan NiuZheng Li
Oct 28, 2019·Frontiers in Plant Science·Dandan LiZheng Li
May 13, 2017·Journal of Experimental Botany·Yun-Song LaiXixiang Li
Apr 23, 2020·Frontiers in Plant Science·Wei-Li GuoGuang-Yin Wang
Mar 20, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yue ChenGang Wang
Feb 13, 2021·The Plant Journal : for Cell and Molecular Biology·Yaqi ZhangXingwang Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
chemical treatments
PCR
dot blotting
reverse transcription-PCRs
chemical treatment

Software Mentioned

Syngene
Gene Tools

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.