A Putative Protein O -Fucosyltransferase Facilitates Pollen Tube Penetration through the Stigma- Style Interface

Plant Physiology
Devin K SmithIan S Wallace

Abstract

During pollen-pistil interactions in angiosperms, the male gametophyte (pollen) germinates to produce a pollen tube. To fertilize ovules located within the female pistil, the pollen tube must physically penetrate specialized tissues. Whereas the process of pollen tube penetration through the pistil has been anatomically well described, the genetic regulation remains poorly understood. In this study, we identify a novel Arabidopsis (Arabidopsis thaliana) gene, O-FUCOSYLTRANSFERASE1 (AtOFT1), which plays a key role in pollen tube penetration through the stigma-style interface. Semi-in vivo growth assays demonstrate that oft1 mutant pollen tubes have a reduced ability to penetrate the stigma-style interface, leading to a nearly 2,000-fold decrease in oft1 pollen transmission efficiency and a 5- to 10-fold decreased seed set. We also demonstrate that AtOFT1 is localized to the Golgi apparatus, indicating its potential role in cellular glycosylation events. Finally, we demonstrate that AtOFT1 and other similar Arabidopsis genes represent a novel clade of sequences related to metazoan protein O-fucosyltransferases and that mutation of residues that are important for O-fucosyltransferase activity compromises AtOFT1 function in vivo. T...Continue Reading

Citations

Jun 26, 2018·Plant Signaling & Behavior·D K SmithI S Wallace
Mar 2, 2019·Journal of Experimental Botany·Ana Lúcia LopesSílvia Coimbra
Nov 15, 2018·International Journal of Molecular Sciences·Yang-Yang ZhengLi-Yu Chen
Mar 4, 2020·Plant Molecular Biology·Prakash B AdhikariRyushiro D Kasahara
Jul 28, 2020·Frontiers in Plant Science·Bussarin WachananawatTakeshi Ishimizu
Jun 3, 2021·Biomolecules·Tibo De ConinckEls J M Van Damme

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