A putative sensor kinase, Hik31, is involved in the response of Synechocystis sp. strain PCC 6803 to the presence of glucose

Microbiology
Shira KahlonAaron Kaplan

Abstract

The reason(s) for glucose sensitivity in certain cyanobacterial strains is poorly understood. Inactivation of genes encoding the putative sensor kinase Hik31 in Synechocystis sp. strain PCC 6803 resulted in a mutant unable to grow in the presence of D-glucose. Sensitivities to D-glucose, its analogue 2-deoxy-D-glucose, and fructose, were alleviated in mutants in which glcP, encoding the glucose transporter, was inactivated. These data indicate that permeation of these substrates is required to inflict cell death. The mutant Deltahik31, and the glucose-sensitive strain of Synechocystis, do not possess glucokinase activity, although a transcript originating from glk, encoding glucokinase, is present. Inactivation of glk led to severe sensitivity to glucose, indicating that the presence of glucose itself, within the cells, inflicted this sensitivity. On the other hand, sensitivity to 2-deoxy-D-glucose was lower in Deltaglk, thus distinguishing between the effect of glucose itself and that of its analogue, which, in the absence of glucokinase activity, may not be phosphorylated. Addition of glucose led to a small rise in glucose-6-phosphate dehydrogenase activity in the wild type, but constitutive activity was observed in the Delta...Continue Reading

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Citations

May 29, 2012·Applied and Environmental Microbiology·Bertram M Berla, Himadri B Pakrasi
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Mark K Ashby, Jean Houmard
Dec 4, 2012·Environment International·Suresh R SubashchandraboseRavi Naidu
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Jan 1, 2013·Metabolites·Doreen SchwarzMartin Hagemann
Jan 17, 2019·PeerJ·José Ángel Moreno-CabezueloJosé Manuel García-Fernández

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