A putrescine-inducible pathway comprising PuuE-YneI in which gamma-aminobutyrate is degraded into succinate in Escherichia coli K-12.

Journal of Bacteriology
Shin KuriharaHideyuki Suzuki

Abstract

Gamma-aminobutyrate (GABA) is metabolized to succinic semialdehyde by GABA aminotransferase (GABA-AT), and the succinic semialdehyde is subsequently oxidized to succinate by succinic semialdehyde dehydrogenase (SSADH). In Escherichia coli, there are duplicate GABA-ATs (GabT and PuuE) and duplicate SSADHs (GabD and YneI). While GabT and GabD have been well studied previously, the characterization and expression analysis of PuuE and YneI are yet to be investigated. By analyzing the amino acid profiles in cells of DeltapuuE and/or DeltagabT mutants, this study demonstrated that PuuE plays an important role in GABA metabolism in E. coli cells. The similarity of the amino acid sequences of PuuE and GabT is 67.4%, and it was biochemically demonstrated that the catalytic center of GabT is conserved as an amino acid residue important for the enzymatic activity in PuuE as Lys-247. However, the regulation of expression of PuuE is significantly different from that of GabT. PuuE is induced by the addition of putrescine to the medium and is repressed by succinate and low aeration conditions; in contrast, GabT is almost constitutive. Similarly, YneI is induced by putrescine, while GabD is not. For E. coli, PuuE is important for utilization o...Continue Reading

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May 23, 2008·The Journal of Biological Chemistry·Shin KuriharaHideyuki Suzuki
Feb 3, 2009·Journal of Bacteriology·Shin KuriharaHideyuki Suzuki

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Citations

May 10, 2011·Applied Microbiology and Biotechnology·Jens Schneider, Volker F Wendisch
Oct 16, 2012·Applied and Environmental Microbiology·Conor FeehilyKimon Andreas G Karatzas
May 29, 2012·Journal of Bacteriology·Barbara L Schneider, Larry Reitzer
Dec 15, 2012·Genome Biology·Christopher J Tervo, Jennifer L Reed
May 16, 2017·BioFactors·Giovana JamarLuciana Pellegrini Pisani
Jan 10, 2017·Journal of Ethnopharmacology·Shuai-Nan ZhangXu-Yan Yang
Oct 17, 2017·Applied and Environmental Microbiology·Claudia Julieta Solís-GonzálezHerminia Loza-Tavera
May 2, 2018·Infection and Immunity·Graham G WillseyMatthew J Wargo
Apr 16, 2015·MBio·Sujatha SrinivasanDavid N Fredricks
Aug 29, 2012·Journal of Applied Microbiology·C Feehily, K A G Karatzas
Apr 11, 2013·The Journal of Biological Chemistry·Shin KuriharaPhilip N Rather
Nov 3, 2016·The Journal of Biological Chemistry·Yuta SugiyamaShin Kurihara
Jun 11, 2019·Frontiers in Bioengineering and Biotechnology·Carsten JersIvan Mijakovic
Jan 18, 2016·Microbial Genomics·Inna A Suvorova, Dmitry A Rodionov
May 8, 2018·Frontiers in Plant Science·Kevin Schwahn, Zoran Nikoloski
Feb 2, 2021·Bioscience of Microbiota, Food and Health·Shin Kurihara
Feb 3, 2021·Journal of Microbiology and Biotechnology·Mihyun JangJong-Hoon Lee

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