A quadrupolar two-photon fluorescent probe for in vivo imaging of amyloid-β plaques

Chemical Science
Cheol Ho HeoHwan Myung Kim

Abstract

The formation of beta amyloid (Aβ) plaques in specific brain regions is one of the early pathological hallmarks of Alzheimer's disease (AD). To enable the early detection of AD and related applications, a method for real-time, clear 3D visualization of Aβ plaques in vivo is highly desirable. Two-photon microscopy (TPM) which utilizes two near-infrared photons is an attractive tool for such applications. However, this technique needs a sensitive and photostable two-photon (TP) probe possessing bright TP exited fluorescence to impart high signal-to-noise (S/N) visualization of Aβ plaques. Herein, we report a quadrupolar TP fluorescent probe (QAD1) having large TP action cross section (Φδmax = 420 GM) and its application for in vivo TPM imaging of Aβ plaques. This probe, designed with a centrosymmetric D-A-D motif with a cyclic conjugating bridge and solubilizing unit, displays bright TP excited fluorescence, appreciable water solubility, robust photostability, and high sensitivity and selectivity for Aβ plaques. Using the real-time TPM imaging of transgenic 5XFAD mice after intravenous injection of QAD1, we show that this probe readily enters the blood brain barrier and provides high S/N ratio images of individual Aβ plaques in v...Continue Reading

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Citations

Mar 19, 2020·International Journal of Molecular Sciences·Christopher D MorroneJoAnne McLaurin
Apr 4, 2020·Organic & Biomolecular Chemistry·Jong Min AnDokyoung Kim
Aug 26, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Aruna K Mora, Sukhendu Nath
Jan 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xi DaiWeiying Lin
Sep 10, 2020·Advanced Materials·Jin ZhouJong Seung Kim
Apr 22, 2021·The Journal of Physical Chemistry Letters·Zoltán Szakács, Eric Vauthey
Mar 6, 2019·ACS Central Science·Yong Woong JunKyo Han Ahn

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Methods Mentioned

BETA
transgenic
fluorescence microscopy

Software Mentioned

Volocity

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