A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.)

Scientific Reports
Kaidong LiuXiaolin Zheng

Abstract

Lysine crotonylation of histone proteins is a recently-identified post-translational modification with multiple cellular functions. However, no information about lysine crotonylation of non-histone proteins in fruit cells is available. Using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity antibody analysis, a global crotonylation proteome analysis of papaya fruit (Carica papaya L.) was performed. In total, 2,120 proteins with 5,995 lysine crotonylation sites were discovered, among which eight conserved motifs were identified. Bioinformatic analysis linked crotonylated proteins to multiple metabolic pathways, including biosynthesis of antibiotics, carbon metabolism, biosynthesis of amino acids, and glycolysis. particularly, 40 crotonylated enzymes involved in various pathways of amino acid metabolism were identified, suggesting a potential conserved function for crotonylation in the regulation of amino acid metabolism. Numerous crotonylation sites were identified in proteins involved in the hormone signaling and cell wall-related pathways. Our comprehensive crotonylation proteome indicated diverse functions for lysine crotonylation in papaya.

References

Feb 8, 2005·Plant Physiology and Biochemistry : PPB·Hamid LazanZainon Mohd Ali
Nov 4, 2005·Angewandte Chemie·Christopher T WalshGregory J Gatto
Nov 7, 2007·Nature Structural & Molecular Biology·John A Latham, Sharon Y R Dent
Oct 31, 2009·Amino Acids·Augusto SorrequietaEstela M Valle
Nov 3, 2010·Journal of Experimental Botany·Juanjuan ZhangCongbing Fang
Jan 28, 2012·Indian Journal of Microbiology·Pedro Chávez-QuintalSantiago Gallegos-Tintoré
May 15, 2013·Journal of the Science of Food and Agriculture·Suphatta Phothiset, Sanguansri Charoenrein
Jun 8, 2013·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Qin GuoXueping Li
Mar 25, 2014·Journal of Experimental Botany·Adriana BastíasJosé A Casaretto
Jul 17, 2014·Journal of Experimental Botany·Rahul KumarArun K Sharma
Dec 17, 2014·Molecular & Cellular Proteomics : MCP·Zhixiang WuYingming Zhao
Jan 20, 2015·Scientific Reports·Weiwei ChenYiguo Hong
Jan 21, 2015·The Plant Journal : for Cell and Molecular Biology·Christopher J SnowdenLee J Sweetlove
Apr 22, 2017·Cell Research·Weizhi XuHongquan Zhang

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Citations

Aug 31, 2020·Proteomics·Jiang-Feng LiuJun-Tao Yang
Sep 3, 2019·Journal of Cellular and Molecular Medicine·Junhu WanHongquan Zhang
Jul 25, 2020·Briefings in Bioinformatics·Xiaoxia MaTian Xie
May 12, 2020·The Plant Journal : for Cell and Molecular Biology·Tao ZhangKaidong Liu
Nov 17, 2020·Frontiers in Microbiology·Ning ZhangMengjie Liu
Feb 8, 2021·Epigenetics & Chromatin·Kun Li, Ziqiang Wang
Jun 2, 2021·Cell & Bioscience·Jia-Yi HouJi-Min Cao
Jul 16, 2021·Cell Death & Disease·Gaoyue JiangHuihui Li

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Datasets Mentioned

BETA
PXD008166

Methods Mentioned

BETA
acetylation

Software Mentioned

WolfPsort
MaxQuant
KEGG mapper
InterProScan
PSORT
Andromeda
KAAS
Motif

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