A quantitative analysis of Arabidopsis plasma membrane using trypsin-catalyzed (18)O labeling.

Molecular & Cellular Proteomics : MCP
Clark J NelsonMichael R Sussman

Abstract

Typical mass spectrometry-based protein lists from purified fractions are confounded by the absence of tools for evaluating contaminants. In this report, we compare the results of a standard survey experiment using an ion trap mass spectrometer with those obtained using dual isotope labeling and a Q-TOF mass spectrometer to quantify the degree of enrichment of proteins in purified subcellular fractions of Arabidopsis plasma membrane. Incorporation of a stable isotope, either H(2)(18)O or H(2)(16)O, during trypsinization allowed relative quantification of the degree of enrichment of proteins within membranes after phase partitioning with polyethylene glycol/dextran mixtures. The ratios allowed the quantification of 174 membrane-associated proteins with 70 showing plasma membrane enrichment equal to or greater than ATP-dependent proton pumps, canonical plasma membrane proteins. Enriched proteins included several hallmark plasma membrane proteins, such as H(+)-ATPases, aquaporins, receptor-like kinases, and various transporters, as well as a number of proteins with unknown functions. Most importantly, a comparison of the datasets from a sequencing "survey" analysis using the ion trap mass spectrometer with that from the quantitati...Continue Reading

References

Oct 1, 1996·Plant Physiology·N D DeWittJ F Harper
Feb 26, 1999·The Plant Journal : for Cell and Molecular Biology·V SantoniM Rossignol
Aug 5, 1999·Biochimie·V SantoniM Rossignol
Apr 29, 2000·Electrophoresis·V SantoniT Rabilloud
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P GygiR Aebersold
Nov 18, 2000·Electrophoresis·T A PrimeP Dupree
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H Shiu, A B Bleecker
Dec 18, 2001·Rapid Communications in Mass Spectrometry : RCM·I I StewartD Figeys
Jun 18, 2002·Plant Physiology·Ildoo HwangJen Sheen
Jan 17, 2003·Plant Physiology·Rainer SchwackeReinhard Kunze
Mar 20, 2003·Journal of Proteome Research·Kristy J ReynoldsCatherine Fenselau
Mar 21, 2003·Journal of Proteome Research·Peiran Liu, Fred E Regnier
Aug 13, 2003·Plant Physiology·Christian DammannJeffrey F Harper
Sep 25, 2003·Journal of Cellular Biochemistry·Tomasz T CalikowskiIris Meier
Oct 11, 2003·The Plant Journal : for Cell and Molecular Biology·Yukio Kawamura, Matsuo Uemura
Dec 13, 2003·Analytical Chemistry·Michael J MacCossJohn R Yates
Mar 30, 2004·Journal of the American Society for Mass Spectrometry·Kenneth L Johnson, David C Muddiman
Apr 3, 2004·Molecular & Cellular Proteomics : MCP·Anne MarmagneGeneviève Ephritikhine
Jun 11, 2004·The Journal of Biological Chemistry·Sébastien MongrandJean-Jacques Bessoule
Jul 28, 2004·Phytochemistry·Sabine BrugièreNorbert Rolland
Aug 6, 2004·Molecular & Cellular Proteomics : MCP·T P J DunkleyK S Lilley
Oct 12, 2004·Journal of Proteome Research·Benjamin J CargileJames L Stephenson
Feb 11, 2005·Current Opinion in Chemical Biology·Michael J MacCoss

❮ Previous
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Citations

Mar 30, 2010·The Journal of Biological Chemistry·Miyoshi HarutaMichael R Sussman
Jan 5, 2012·Molecular & Cellular Proteomics : MCP·James Mitch ElmoreGitta Coaker
Jan 10, 2008·Molecular & Cellular Proteomics : MCP·Wenqiang TangZhi-Yong Wang
Dec 26, 2012·Antioxidants & Redox Signaling·Sabine LüthjeKerstin Wöltje
Nov 16, 2010·Plant Physiology·Hiren J JoshiJoshua L Heazlewood
Dec 7, 2007·The Plant Cell·Jay J Thelen, Scott C Peck
May 14, 2010·Annual Review of Biophysics·Kelli G Kline, Michael R Sussman
Dec 6, 2012·International Journal of Plant Genomics·Cosette AbdallahKjell Sergeant
Mar 3, 2010·Annual Review of Plant Biology·Waltraud X Schulze, Björn Usadel
Feb 10, 2007·Expert Review of Proteomics·Xian ChenPengyuan Yang
Apr 7, 2007·FEBS Letters·Roberto A GaxiolaKarin Schumacher
May 1, 2007·FEBS Letters·Doris RentschMechthild Tegeder
Oct 30, 2012·Clinical Biochemistry·Leroi V DeSouza, K W Michael Siu
May 28, 2011·Trends in Biochemical Sciences·Jeffrey N SavasJohn R Yates
Mar 1, 2011·Journal of Proteomics·A VertommenS C Carpentier
Mar 4, 2011·Phytochemistry·Uma Kota, Michael B Goshe
Nov 3, 2009·Progress in Lipid Research·Jacques JoyardNorbert Rolland
Mar 8, 2016·Biochimica Et Biophysica Acta·Xiaobai LiSheng Zhang
Mar 22, 2014·The Protein Journal·Fernanda R RabelloAngela Mehta
Jun 25, 2009·The New Phytologist·Simonetta Santi, Wolfgang Schmidt
Aug 31, 2007·The Plant Journal : for Cell and Molecular Biology·Holger EubelA Harvey Millar
Sep 10, 2008·Proteomics·Pawel G SadowskiKathryn S Lilley
Nov 7, 2006·Mass Spectrometry Reviews·Masaru Miyagi, K C Sekhar Rao
Jul 19, 2008·Mass Spectrometry Reviews·Xiaojun FengBi-Feng Liu
May 26, 2010·The Plant Journal : for Cell and Molecular Biology·Norbert MehlmerMarkus Teige
Nov 3, 2010·Mass Spectrometry Reviews·Ganesh Kumar AgrawalRandeep Rakwal
Jan 20, 2011·Proteomics·Laurence V Bindschedler, Rainer Cramer
Jul 15, 2009·Mass Spectrometry Reviews·Xinyu LiuYu-Quan Wei
Feb 3, 2015·Proteomics·Xianping FangChengqi Yan
Nov 1, 2007·Plant Signaling & Behavior·Fabienne FurtSébastien Mongrand
Nov 19, 2014·Journal of Agricultural and Food Chemistry·Xiao-Mei ShaHui Xiao
Sep 10, 2016·Current Opinion in Biotechnology·Dana M Freund, Adrian D Hegeman
Dec 9, 2010·Molecular Plant-microbe Interactions : MPMI·Iris K Jarsch, Thomas Ott
Jun 26, 2007·Molecular & Cellular Proteomics : MCP·Totte NiittyläWaltraud X Schulze

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