PMID: 11342965May 9, 2001Paper

A quantitative analysis of purinoceptor expression in human fetal and adult bladders

The Journal of Urology
Barry A O'ReillyStephen B McMahon

Abstract

In adults there is evidence that adenosine triphosphate acting at P2X receptors functions as a co-transmitter at vesical smooth muscle. The contractile mechanisms of human fetal bladder have been studied to a limited extent and it remains undetermined whether P2X receptors contribute. We compared the expression of the 7 known P2X receptors in fetal and adult human bladders using a quantitative polymerase chain reaction (PCR) based method. Real-time quantitative reverse transcriptase-PCR provides a system for the detection and analysis of RNA. Four complete cadaver fetal bladders were obtained at 16 weeks to full-term gestation and divided into a total of 12 segments. Adult bladder samples were obtained from 4 patients requiring bladder biopsy. Total RNA was extracted from each sample and 10 ng. were used for individual PCR reactions. An ABI 7700 machine (PE Applied Biosystems, California) determined expression levels of the 7 P2X genes in total RNA. In adult bladders P2X1 was by far the predominant purinergic receptor at the messenger RNA level. The remaining purinergic receptors were consistently present in the order P2X1 > P2X4 > P2X7 > P2X5 > P2X2 > P2X3 = P2X6 = 0. In fetal bladders the expression of P2X1 transcripts was mu...Continue Reading

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Citations

Dec 25, 2007·Pflügers Archiv : European journal of physiology·Melinda WuestUrsula Ravens
Dec 10, 2003·Urology·Karl Erik Andersson
Dec 18, 2001·The Journal of Urology·Barry A O'ReillyStephen B McMahon
Dec 21, 2002·Urology·Karl-Erik Andersson, Petter Hedlund
Aug 7, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Melih TugayFaruk Erden
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Mar 7, 2014·Canadian Journal of Physiology and Pharmacology·Olivier ThériaultMohamed Chahine
Apr 12, 2008·Purinergic Signalling·Elena AdinolfiDavide Ferrari
Jan 25, 2008·American Journal of Physiology. Renal Physiology·Bikramjit ChopraLori A Birder
Jan 31, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiqun YuWarren G Hill
Jan 12, 2011·Cellular and Molecular Life Sciences : CMLS·Geoffrey Burnstock, Henning Ulrich
Oct 22, 2005·Current Urology Reports·William I Jaffe, Alexis E Te
Nov 24, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Naoki YoshimuraPradeep Tyagi
Oct 20, 2010·The Urologic Clinics of North America·J Quentin Clemens
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Feb 9, 2006·British Journal of Pharmacology·Anthony P D W FordDebra A Cockayne
Apr 22, 2010·Basic & Clinical Pharmacology & Toxicology·Patrik AronssonDaniel Giglio
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May 9, 2015·Autonomic Neuroscience : Basic & Clinical·Cristina Giaroni
May 21, 2015·Purinergic Signalling·Geoffrey Burnstock, Nicholas Dale
Aug 9, 2015·BMC Urology·Karl SvennerstenMirjana Poljakovic
Jul 17, 2012·The American Journal of Pathology·Shu ZhangCong-Yi Wang
Jul 3, 2013·Biochemical and Biophysical Research Communications·Romel WazirKun-Jie Wang
Apr 15, 2008·Journal of Pediatric Surgery·Melih TugayTijen Utkan
Feb 3, 2018·Journal of Cellular and Molecular Medicine·Na N GuanKarl Svennersten
Jan 31, 2012·Urologia·Emilio Sacco
Nov 13, 2004·The Journal of Urology·Yasuhiko IgawaKarl-Erik Andersson
Feb 3, 2005·American Journal of Physiology. Renal Physiology·Guiming Liu, Firouz Daneshgari
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Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock

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