A quantitative analysis of the glomerular charge barrier in the rat

American Journal of Physiology. Renal Physiology
J SörenssonBörje Haraldsson


Modifying the ionic strength (I) is a gentle way to alter charge interactions, but it cannot be done for studies of the glomerular sieving of proteins in vivo. We therefore perfused 18 isolated rat kidneys with albumin solutions of different ionic strengths at a low temperature (cIPK) to inhibit tubular uptake and protease activity. Four anionic proteins were studied, namely albumin (Alb), orosomucoid (Oro), ovalbumin (Ova), and anionic horseradish peroxidase (aHRP), together with the neutral polymer Ficoll. With normal ionic strength of the perfusate (152 mM), the fractional clearance (theta) was 0.0018 +/- 0.0003 for Alb, 0.0033 +/- 0.0003 for Oro, 0.090 +/- 0.008 for Ova, and 0.062 +/- 0.002 for aHRP. These theta values were all lower than for Ficoll of similar hydrodynamic size; e.g., theta(Ficoll 36 A) was >20 times higher than theta for albumin. Low ionic strength (34 mM) increased size selectivity as theta for anionic proteins and Ficoll fell, suggesting a reduction in small-pore radius from 44 +/- 0.4 to 41 +/- 0.5 A, P < 0.01. In contrast, low I reduced the charge density of the membrane, omega, to one-quarter of the 20--50 meq/l estimated at normal I. These dynamic changes in omega seem to be due to volume alterations...Continue Reading


Oct 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H G Rennke, M A Venkatachalam
Jan 1, 1978·The Journal of Clinical Investigation·M P BohrerB M Brenner
Feb 1, 1992·Kidney International·Börje HaraldssonB Rippe
Jan 1, 1992·Acta Physiologica Scandinavica·E K Johnsson, Börje Haraldsson
Mar 1, 1987·Acta Physiologica Scandinavica·G OjtegM Wolgast
Feb 1, 1971·Archives of Biochemistry and Biophysics·J S CharnockJ C Russell
Dec 1, 1974·Biophysical Journal·J L Anderson, D M Malone
Nov 1, 1981·The American Journal of Physiology·M J Hanley, K Davidson
May 1, 1982·The Journal of Cell Biology·Y S Kanwar, L J Rosenzweig
Feb 1, 1980·The American Journal of Physiology·W M DeenJ M Jamieson
Jul 1, 1980·Microvascular Research·F E Curry, C C Michel
May 1, 1995·Kidney International·Wayne D Comper, E F Glasgow
Nov 1, 1993·The Journal of Clinical Investigation·A GuaschB D Myers
Jan 1, 1994·Physiological Reviews·Bengt Rippe, Börje Haraldsson
Jun 5, 1998·The American Journal of Physiology·G R BoltonB S Daniels
Jul 2, 1998·Acta Physiologica Scandinavica·J SörenssonBörje Haraldsson
Aug 1, 1998·Biochimica Et Biophysica Acta·T M Osicka, Wayne D Comper
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RuotsalainenK Tryggvason
Jul 15, 2000·American Journal of Physiology. Renal Physiology·M OhlsonBörje Haraldsson
Feb 22, 2001·American Journal of Physiology. Renal Physiology·M OhlsonBörje Haraldsson
Mar 1, 1936·The Biochemical Journal·O Lamm, A Polson


Jan 25, 2008·Journal of the American Society of Nephrology : JASN·Wayne D ComperWilliam M Deen
Apr 19, 2015·Current Opinion in Nephrology and Hypertension·Turgay SaritasMarcus J Moeller
Nov 6, 2001·American Journal of Physiology. Renal Physiology·Wayne D Comper
Dec 12, 2001·American Journal of Physiology. Renal Physiology·Clarice Kazue FujiharaRoberto Zatz
Jul 24, 2003·American Journal of Physiology. Renal Physiology·Marco A M GuimarãesWayne D Comper
Mar 3, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Rolf E F ChristiansenBjarne M Iversen
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Jenny SörenssonBörje Haraldsson
May 23, 2012·Current Opinion in Nephrology and Hypertension·Ralf HausmannMarcus J Moeller
Aug 25, 2016·Cellular and Molecular Bioengineering·Matthew A DragovichX Frank Zhang
Aug 16, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Paul M A van HaarenJos A E Spaan
Dec 9, 2004·American Journal of Physiology. Renal Physiology·Anna BjörnsonJenny Sörensson
Apr 18, 2009·Current Opinion in Nephrology and Hypertension·George Jarad, Jeffrey H Miner
Feb 13, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Bingmei M Fu, John M Tarbell
Mar 27, 2013·Nature Reviews. Nephrology·Marcus J Moeller, Verena Tenten
Feb 18, 2017·Current Opinion in Nephrology and Hypertension·Sara ContiTobias B Huber
Aug 15, 2001·American Journal of Physiology. Renal Physiology·Clara HjalmarssonBörje Haraldsson
Jun 19, 2003·American Journal of Physiology. Renal Physiology·Giuliano CiarimboliBörje Haraldsson
Jan 24, 2004·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Börje Haraldsson, Jenny Sörensson
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Ulla LundBengt Rippe
Dec 17, 2008·Journal of the American Society of Nephrology : JASN·Marie JeanssonBörje Haraldsson
Oct 30, 2010·Journal of the American Society of Nephrology : JASN·Elena GagliardiniAndrea Remuzzi
Aug 11, 2005·American Journal of Physiology. Renal Physiology·Marie Jeansson, Börje Haraldsson
Dec 31, 2002·Physiological Reviews·Hermann PavenstädtMatthias Kretzler
Apr 9, 2008·Physiological Reviews·Börje HaraldssonWilliam M Deen

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