PMID: 8972870Dec 1, 1996Paper

A quantitative and specific method for measuring transcript levels of highly homologous genes

Nucleic Acids Research
A J Lombardo, G B Brown

Abstract

Because of their high nucleotide sequence homology, the specific detection of mRNA transcripts of individual members of a gene family presents certain problems. Here we apply and defend the single nucleotide primer extension (SNuPE) as a simple, specific, and highly quantitative assay for this purpose. The method distinguishes regions of the brain sodium channel gene family that vary by as little as a single nucleotide. The technique has been modified to include an intersample normalizer, and adaptation of the SNuPE assay to other gene families of interest to neuroscientists should be easy to achieve.

References

Jan 15, 1992·Biochemical and Biophysical Research Communications·F H LinK Iqbal
Nov 4, 1992·JAMA : the Journal of the American Medical Association·J M BertoniD C Gajdusek
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M N KuppuswamyS P Bajaj
Feb 1, 1990·Brain Research. Molecular Brain Research·R H JohoA M Brown
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G MandelP Brehm
Feb 1, 1994·PCR Methods and Applications·J Singer-Sam
Jan 1, 1994·Annual Review of Neuroscience·R L Macdonald, R W Olsen
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