A quantitative approach to characterizing cell lysis caused by mechanical agitation of Streptomyces clavuligerus

Biotechnology Progress
Johannes A RoubosJoseph J Heijnen

Abstract

Streptomyces clavuligerus is a commercially important actinomycete that is used to produce clavulanic acid, a beta-lactamase inhibitor. Observations during 10 batch cultivations with S. clavuligerus on defined media have led to the finding that the organism is very sensitive to shear when grown in batch cultures with increasing stirrer speed. The stirrer speed was increased to keep the dissolved oxygen level above 50% air saturation. A quantitative approach based on the calculation of elemental balances and a simple mathematical model is proposed to characterize the biomass lysis. Finally, a linear relation between biomass yield and observed specific growth rate is determined. Results show that cell lysis occurs at a high degradation rate, e.g., mu(max) = 0.16 h(-1) and k(d) = 0.07 h(-1), when the gassed power input increases above 1.1, 1.7, or 2.0 kW/m(3), respectively, depending on the medium composition. The overall biomass yield on substrate is dramatically reduced in all experiments (>30%).

References

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Citations

Aug 28, 2012·Bioprocess and Biosystems Engineering·E OlmosS Delaunay
Mar 3, 2010·Analytical Chemistry·Yong ZengRichard A Mathies
Aug 4, 2006·Applied and Environmental Microbiology·Gilles P van WezelBarend Kraal
Jan 15, 2014·Applied Microbiology and Biotechnology·Tiago BeitesMarta V Mendes
May 3, 2008·Biotechnology Advances·Parag S SaudagarRekha S Singhal
Apr 19, 2015·Microbial Cell Factories·Dino van DisselGilles P van Wezel
Sep 15, 2016·Biotechnology Journal·Ruben R G SoaresM Raquel Aires-Barros
Nov 3, 2017·Antonie van Leeuwenhoek·Dino van Dissel, Gilles P van Wezel
Jan 26, 2021·3 Biotech·Stefanie B Costa-GutierrezMarta A Polti

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