PMID: 3322261Oct 15, 1987Paper

A quantitative approach to sequence comparisons of nitrogenase MoFe protein alpha- and beta-subunits including the newly sequenced nifK gene from Klebsiella pneumoniae

The Biochemical Journal
D HollandJ L Sussman

Abstract

The nucleotide sequence was determined for part of the Klebsiella pneumoniae nif gene cluster containing the 3' end of the nifD gene and the entire length of the nifK gene (encoding the alpha- and beta-subunits of the nitrogenase MoFe protein respectively), as well as the putative start of the nifY gene, a gene of as yet unknown function. A broad-based comparison of a number of MoFe protein alpha-subunits, beta-subunits and alpha-versus beta-subunits was carried out by the use of a computer program that simultaneously aligns three protein sequences according to the mutation data matrix of Dayhoff. A new kind of quantitative statistical measure of the similarity between the aligned sequences was obtained by calculating and plotting standardized similarity scores for overlapping segments along the aligned proteins. This calculation determines if a test sequence is similar to the consensus sequence of two other proteins that are known to be related to each other. The different beta-subunits compared were found to be significantly similar along most of their sequence, with the exception of two relatively short regions centred around residues 225 and 300, which contain insertions/deletions. The overall pattern of similarity between ...Continue Reading

Citations

Jul 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Leonardo Curatti, Luis M Rubio
Mar 1, 1990·Human Nature : an Interdisciplinary Biosocial Perspective·M Daly, M Wilson
Jan 1, 1990·Critical Reviews in Microbiology·S M Hinton, D Dean
Feb 1, 1989·Journal of Bacteriology·M R JacobsonD R Dean

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