PMID: 7086151Jan 1, 1982Paper

A quantitative assay for IgA rheumatoid factor

Journal of Immunological Methods
W J KoopmanA Solomon

Abstract

We developed a solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgA rheumatoid factor (RF) in biological fluids. Human IgM RF, IgG RF, IgG, IgA, IgM and whole serum did not significantly interfere with the IgA RF assay. Patients with sero-positive rheumatoid arthritis (RA) had significantly higher concentrations of IgA RF than sero-negative RA patients or healthy adult controls. Concentrations of IgA RF in paired sera and synovial fluids from sero-positive RA patients were comparable. Levels of IgA RF demonstrated a moderately good correlation with levels of IgM RF in sero-positive RA sera (r = 0.673). However, the ratio of IgA RF concentration to IgM RF concentration in sero-positive RA sera varied widely.

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C Cunningham-RundlesN K Day
Jan 1, 1978·Arthritis and Rheumatism·D J StankaitienéJ P Jusénaité
Jul 1, 1979·The Journal of Clinical Investigation·C Cunningham-RundlesN K Day
Jan 1, 1980·The Journal of Experimental Medicine·J L van Snick, P L Masson
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Dec 1, 1964·The Journal of Experimental Medicine·R E SchrohenloherT B TOMASI

Citations

Sep 1, 1988·Journal of Clinical Immunology·F H SennhauserD M Roberton
Sep 1, 1988·Journal of Clinical Immunology·S JacksonW J Koopman
May 1, 1991·Journal of Clinical Immunology·J Thé, J L Ebersole
Jan 1, 1987·Annals of the Rheumatic Diseases·M Gioud-PaquetJ C Monier
Nov 1, 1987·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·B A JulianS Jackson
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·M A van LeeuwenM H van Rijswijk
Jun 30, 1983·Annals of the New York Academy of Sciences·W J KoopmanR E Schrohenloher

Related Concepts

Antibody Specificity
Rheumatoid Arthritis
Binding Sites, Antibody
IgA2
Polyglobin
IgM2
Fab Immunoglobulins
Immunoglobulin Fc Fragments
Rheumatoid Factor
Synovial Fluid

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