A quantitative assay for subclassing IgG alloantibodies implicated in hemolytic disease of the newborn

Vox Sanguinis
N C ThomasT S Kickler

Abstract

Traditionally, IgG subclassing has been performed using qualitative assays. Quantitation of IgG subclasses may have prognostic value in evaluating alloimmunized pregnancies. A quantitative enzyme-linked immunosorbent assay (ELISA) was implemented for measuring IgG subclasses of red blood cell (RBC) antibodies (AB) isolated by adsorption/elution from the sera of alloimmunized pregnant women. The assay is a sandwich enzyme immunoassay using monoclonal antibodies specific for the relevant IgG subclasses and anti-human IgG peroxidase conjugate to quantitate the amount of bound IgG. The sensitivities of the assay for IgG1, 2, 3, and 4, respectively, were 4, 23, 4 and 2 micrograms/l. The results for each subclass for a given AB were expressed as a percentage of the total. In a series of pregnant mothers with ABs: E (4), Fya (2), Jka (1) and S (1), the mean percentage +/- 1SD of each subclass was: IgG1 61 +/- 34; IgG2 14 +/- 22; IgG3 18 +/- 28 and IgG4 4 +/- 17. IgG1 or IgG3 accounted for greater than 50% of the AB subclass distribution in 5 cases that resulted in hemolytic disease of the newborn (HDN). Although only a small number of samples was studied, changes in the concentrations of IgG1 or IgG3 during gestation suggest a correla...Continue Reading

References

May 1, 1978·Acta Physiologica Scandinavica·S BlomstrandI Kjellmer
Sep 1, 1989·Immunology Letters·P D MehtaC E Isaacs
Jun 1, 1986·American Journal of Obstetrics and Gynecology·M M TaslimiJ V Dacus
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Feb 1, 1986·Obstetrics and Gynecology·J A CopelJ C Hobbins

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