A quantitative description of tubular system Ca(2+) handling in fast- and slow-twitch muscle fibres

The Journal of Physiology
Tanya R CullyB S Launikonis

Abstract

Current methods do not allow a quantitative description of Ca(2+) movements across the tubular (t-) system membrane without isolating the membranes from their native skeletal muscle fibre. Here we present a fluorescence-based method that allows determination of the t-system [Ca(2+) ] transients and derivation of t-system Ca(2+) fluxes in mechanically skinned skeletal muscle fibres. Differences in t-system Ca(2+) -handling properties between fast- and slow-twitch fibres from rat muscle are resolved for the first time using this new technique. The method can be used to study Ca(2+) handling of the t-system and allows direct comparisons of t-system Ca(2+) transients and Ca(2+) fluxes between groups of fibres and fibres from different strains of animals. The tubular (t-) system of skeletal muscle is an internalization of the plasma membrane that maintains a large Ca(2+) gradient and exchanges Ca(2+) between the extracellular and intracellular environments. Little is known of the Ca(2+) -handling properties of the t-system as the small Ca(2+) fluxes conducted are difficult to resolve with conventional methods. To advance knowledge in this area we calibrated t-system-trapped rhod-5N inside skinned fibres from rat and [Ca(2+) ]t-sys ,...Continue Reading

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Citations

Apr 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Rocky H ChoiBradley S Launikonis
Aug 25, 2019·Journal of Muscle Research and Cell Motility·Mudassir Haider RizviArifa Savanur
Jul 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tanya R CullyBradley S Launikonis
Oct 3, 2018·Communications Biology·Xaver KoenigBradley S Launikonis
Dec 15, 2020·The Journal of General Physiology·Aldo Meizoso-Huesca, Bradley S Launikonis
Jun 9, 2020·Redox Biology·Tanya R Cully, George G Rodney
Feb 18, 2021·Journal of Muscle Research and Cell Motility·D Mejía-RaigosaJ C Calderón
Feb 18, 2021·American Journal of Physiology. Cell Physiology·Daiki Watanabe, Masanobu Wada
Jun 4, 2021·Biochimica Et Biophysica Acta. General Subjects·Andrés F MilánJuan C Calderón

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