PMID: 8963723May 1, 1996Paper

A quantitative immunoassay for the lysine-binding function of lipoprotein(a). Application to recombinant apo(a) and lipoprotein(a) in plasma

Arteriosclerosis, Thrombosis, and Vascular Biology
J L Hoover-PlowE F Plow

Abstract

Apo(a), the unique apoprotein of lipoprotein(a) (Lp[a]), can express lysine-binding sites(s) (LBS). However, the LBS activity of Lp(a) is variable, and this heterogeneity may influence its pathogenetic properties. An LBS-Lp(a) immunoassay has been developed to quantitatively assess the LBS function of Lp(a). Lp(a) within a sample is captured with an immobilized monoclonal antibody specific for apo(a), and the captured Lp(a) is reacted with an antibody specific for functional LBS. The binding of this LBS-specific antibody is then quantified by using an alkaline phosphatase-conjugated disclosing antibody. The critical LBS-specific antibody was raised to kringle 4 of plasminogen. When applied to plasma samples, the LBS activity of Lp(a) ranged from 0% to 100% of an isolated reference Lp(a); the signal corresponded to the percent retention of Lp(a) on a lysine-Sepharose but did not correlate well with total Lp(a) levels in plasma. Mutation of residues in the putative LBS in the carboxy-terminal kringle 4 repeat (K4-37) in an eight-kringle apo(a) construct resulted in marked but not complete loss of activity in the LBS-Lp(a) immunoassay. These data suggest that this kringle is the major but not the sole source of LBS activity in apo...Continue Reading

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Citations

Jun 24, 1998·Biochimica Et Biophysica Acta·J Hoover-Plow, P Skocir
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·R M LawnL Patthy
Aug 1, 1997·The Journal of Clinical Investigation·N W BoonmarkR M Lawn
Sep 10, 2005·Journal of Thrombosis and Haemostasis : JTH·J ShaJ Hoover-Plow
Mar 31, 1998·Clinical Genetics·N W Boonmark, R M Lawn

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