DOI: 10.1101/489658Dec 7, 2018Paper

A quantitative inventory of yeast P body proteins reveals principles of compositional and specificity

BioRxiv : the Preprint Server for Biology
Wenmin XingMichael K Rosen


P bodies are archetypal biomolecular condensates that concentrate proteins and RNA without a surrounding membrane. While dozens of P body proteins are known, the concentrations of components in the compartment have not been measured. We used live cell imaging to generate a quantitative inventory of the major proteins in yeast P bodies. Only 7 proteins are highly concentrated in P bodies (5.1-15 uM); the 24 others examined are appreciably lower (most ≤ 2.6 uM). P body concentration correlates inversely with cytoplasmic exchange rate. Sequence elements driving Dcp2 concentration into P bodies are distributed across the protein and act cooperatively. Our data indicate that P bodies, and probably other condensates, are biochemically simpler than suggested by proteomic analyses, with implications for specificity, reconstitution and evolution.

Related Concepts

Body Fluid Compartments
Biological Evolution
High Mobility Group Proteins
Placental Tissue Sample
DCP2 protein, human
Body Structure

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