A rapid, inexpensive, and quantitative method is described for obtaining the levels of plasma very low, low, and high density lipoproteins using cellulose acetate electrophoresis and lipid assays without prior separation by ultracentrifuge or other techniques. It involves separation of the lipoproteins by cellulose acetate electrophoresis, followed by their identification with the ozone-Schiff reaction. The total lipoprotein concentration is estimated from the total plasma phospholipid, and the percentage of each component obtained by densitometric analysis of the stained electrophoretograms, using reflected light. For samples with a raised level of very low density lipoprotein, plasma triglyceride analysis is also required. The results obtained by the cellulose acetate electrophoresis method are in good agreement with those by the analytical ultracentrifuge and the preparative ultracentrifuge with refractometry. The theoretical assumptions on which the method is based have been shown to be valid.
Separation and quantitative analysis of serum lipoproteins by means of electrophoresis on cellulose acetate
Studies on the phenotyping of hyperlipoproteinemias. Evaluation of cellulose acetate technique and comparison with paper electrophoresis
Diet and serum lipids in atherosclerotic coronary heart disease. Etiologic and preventive considerations
Evaluation of the discriminative power of lipidophoresis by discriminant analysis using a simple quantitative method of agarose lipidophoresis
Lipoprotein(a) serum concentration and apolipoprotein(a) phenotype correlate with severity and presence of ischemic cerebrovascular disease
Genomic compatibility occurs over a wide range of parental genetic similarity in an outcrossing plant
Host-based divergence in populations of the pea aphid: insights from nuclear markers and the prevalence of facultative symbionts
Daphnia hybridization along ecological gradients in pelagic environments: the potential for the presence of hybrid zones in plankton
Free cholesterol transfer from human lower-density lipoproteins (d less than 1.063) to lipoprotein-deficient serum and high-density lipoproteins
ApoE, Lipids & Cholesterol
Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.