A Quantitative Proteomic Analysis of In Vitro Assembled Chromatin.

Molecular & Cellular Proteomics : MCP
Moritz Carl Völker-AlbertAxel Imhof

Abstract

The structure of chromatin is critical for many aspects of cellular physiology and is considered to be the primary medium to store epigenetic information. It is defined by the histone molecules that constitute the nucleosome, the positioning of the nucleosomes along the DNA and the non-histone proteins that associate with it. These factors help to establish and maintain a largely DNA sequence-independent but surprisingly stable structure. Chromatin is extensively disassembled and reassembled during DNA replication, repair, recombination or transcription in order to allow the necessary factors to gain access to their substrate. Despite such constant interference with chromatin structure, the epigenetic information is generally well maintained. Surprisingly, the mechanisms that coordinate chromatin assembly and ensure proper assembly are not particularly well understood. Here, we use label free quantitative mass spectrometry to describe the kinetics of in vitro assembled chromatin supported by an embryo extract prepared from preblastoderm Drosophila melanogaster embryos. The use of a data independent acquisition method for proteome wide quantitation allows a time resolved comparison of in vitro chromatin assembly. A comparison of...Continue Reading

References

Nov 1, 1978·Cell·A WorcelM L Wong
Mar 27, 1984·Biochemistry·P A SmithR Chalkley
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BulgerJ T Kadonaga
May 1, 1997·Methods : a Companion to Methods in Enzymology·T A BlankP B Becker
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·B LadouxJ L Viovy
Oct 3, 2001·Journal of Structural Biology·A Y DunnJ Frydman
Dec 28, 2002·Genes & Development·Matthew G GuentherMitchell A Lazar
Aug 16, 2005·Biophysical Journal·Gaudeline WagnerJean-Louis Viovy
Feb 9, 2006·The Journal of Biological Chemistry·Laura J BensonAnthony T Annunziato
Oct 21, 2006·Molecular Cell·Alejandra LoyolaGeneviève Almouzni
Dec 22, 2007·Science·Anja GrothGeneviève Almouzni
Nov 13, 2008·Molecular and Cellular Biology·Annette N D ScharfAxel Imhof
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jun 23, 2009·Nucleic Acids Research·Annette N D ScharfAxel Imhof
Oct 19, 2010·Nature Structural & Molecular Biology·Eric I CamposDanny Reinberg
Jan 25, 2011·Nature Biotechnology·Marcus BantscheffGerard Drewes
Feb 2, 2011·PloS One·Sebastian RealAlbert Tauler
Apr 2, 2011·The Journal of Biological Chemistry·Francisca AlvarezAlejandra Loyola
Sep 22, 2011·PloS One·Mariana P TorrenteBenjamin A Garcia
Nov 17, 2011·Genes & Development·Sheila S Teves, Steven Henikoff
Feb 23, 2012·Scientific Reports·Anna E KliszczakCorrado Santocanale
Apr 12, 2013·ELife·Mark HedglinStephen Benkovic
Jul 16, 2013·ELife·Sarah H ShahmoradianWah Chiu
Aug 8, 2013·Nature Methods·Dattatreya MellacheruvuAlexey I Nesvizhskii
Jan 25, 2014·Biochimica Et Biophysica Acta·Ali Hamiche, Muhammad Shuaib
Feb 19, 2014·The EMBO Journal·Georg KustatscherJuri Rappsilber
Feb 13, 2015·Nature Protocols·Olga T SchubertRuedi Aebersold
Mar 21, 2015·Genes & Development·Constance AlabertAnja Groth

❮ Previous
Next ❯

Citations

Jun 23, 2016·Data in Brief·Moritz Carl Völker-AlbertAxel Imhof
Oct 28, 2019·Proteomics·Alberto Valdés, Sara Bergström Lind
May 31, 2019·Nucleic Acids Research·Lisa HarpprechtPeter B Becker
Oct 26, 2021·Frontiers in Cell and Developmental Biology·Hongyu ZhaoLu Cai

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.