A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labelling with amino acids in cell culture.

Proteomics
Brian K DoveJulian A Hiscox

Abstract

Influenza A virus is one of the world's major uncontrolled pathogens, causing seasonal epidemics as well as global pandemics. This was evidenced by the recent emergence and now prevalence of the 2009 swine origin pandemic H1N1 influenza A virus. In this study, quantitative proteomics using stable isotope labelling with amino acids in cell culture was used to investigate the changes in the host cell proteome in cells infected with pandemic H1N1 influenza A virus. The study was conducted in A549 cells that retain properties similar to alveolar cells. Several global pathways were affected, including cell cycle regulation and lipid metabolism, and these could be correlated with recent microarray analyses of cells infected with influenza A virus. Taken together, both quantitative proteomics and transcriptomic approaches can be used to identify potential cellular proteins whose functions in the virus life cycle could be targeted for chemotherapeutic intervention.

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Citations

May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chen YangEckard Wimmer
Jun 11, 2014·Journal of Proteomics·Anja M BillingClaude P Muller
Aug 14, 2015·Journal of Analytical Science and Technology·Youri LeeSang-Moo Kang
May 29, 2014·Journal of Lipid Research·Lukas Bahati TannerMarkus R Wenk
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yutong SongEckard Wimmer
Jun 27, 2014·Journal of Virology·Aitor NogalesLuis Martínez-Sobrido
Nov 16, 2018·MSphere·Grant BeyleveldMegan L Shaw
Jan 29, 2017·The Journal of Biological Chemistry·Ying FanSumana Sanyal
Jul 3, 2021·Proteomes·Eleni I KatsarouAngeliki I Katsafadou

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