A quantitative proteomic approach for identification of potential biomarkers in hepatocellular carcinoma.

Journal of Proteome Research
Raghothama ChaerkadyPaul J Thuluvath

Abstract

Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. In this study, our objective was to identify differentially regulated proteins in HCC through a quantitative proteomic approach using iTRAQ. More than 600 proteins were quantitated of which 59 proteins were overexpressed and 92 proteins were underexpressed in HCC as compared to adjacent normal tissue. Several differentially expressed proteins were not implicated previously in HCC. A subset of these proteins (six each from upregulated and downregulated groups) was further validated using immunoblotting and immunohistochemical labeling. Some of the overexpressed proteins with no previous description in the context of HCC include fibroleukin, interferon induced 56 kDa protein, milk fat globule-EGF factor 8, and myeloid-associated differentiation marker. Interestingly, all the enzymes of urea metabolic pathway were dramatically downregulated. Immunohistochemical labeling confirmed differential expression of fibroleukin, myeloid associated differentiation marker and ornithine carbamoyl transferase in majority of HCC samples analyzed. Our results demonstrate quantitative proteomics as a robust discovery tool for the identification of differentially regulated pr...Continue Reading

References

Apr 1, 1996·Journal of Gastroenterology and Hepatology·Y SuzukiH Asakura
Feb 9, 1996·Molecular and Cellular Biochemistry·R Makino, M Yamaguchi
Aug 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S NakamuraA S Syed
Jul 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Moritoshi Kinoshita, Masahiko Miyata
Aug 16, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michael TorbensonJohn Boitnott
Apr 3, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Rajesh KannangaiMichael Torbenson
Apr 13, 2004·Molecular & Cellular Proteomics : MCP·Steven CarrUNKNOWN Working Group on Publication Guidelines for Peptide and Protein Identification Data
Apr 24, 2004·The American Journal of Surgical Pathology·Lisa M YerianJohn Hart
Jul 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ethan D EmberleyPeter H Watson
Oct 28, 2004·Gastroenterology·Hashem B El-Serag
Jan 22, 2005·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Feb 1, 2005·Journal of Virology·Andreas DornWalter Doerfler
Feb 4, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Valérie ParadisPierre Bedossa
Feb 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E Ellen SchweglerRichard R Drake
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qin NingXiaoping Luo
Aug 4, 2005·Journal of the National Cancer Institute·Mirjana UrosevicMichel Gilliet
Dec 31, 2005·Nucleic Acids Research·Minoru KanehisaMika Hirakawa
Jun 13, 2006·Proteomics·Motonari TakashimaKazuyuki Nakamura
Aug 10, 2006·Proteomics·Jeremy E MelansonDevanand M Pinto
Aug 31, 2006·Expert Opinion on Emerging Drugs·Lewis R Roberts, Gregory J Gores
Oct 25, 2006·British Journal of Cancer·D G WardP J Johnson
Dec 21, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Fahd Al-MullaWalter Kolch
Mar 16, 2007·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Jul 10, 2007·The American Journal of Gastroenterology·Richard K SterlingMyron E Schwartz
Sep 15, 2007·Molecular & Cellular Proteomics : MCP·Yunhao ChenYoon-Pin Lim
Feb 9, 2008·Nature Biotechnology·Suresh MathivananAkhilesh Pandey
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Apr 7, 2009·Journal of the American Society for Mass Spectrometry·Jenny M ArmentaIulia M Lazar
May 21, 2010·Journal of the American Society for Mass Spectrometry·Min-Sik KimAkhilesh Pandey
Oct 12, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Anna KakehashiHideki Wanibuchi
Apr 8, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jianzhong AiQin Zhou
May 17, 2011·Thrombosis Journal·Qila Sa, Jane L Hoover-Plow
Dec 4, 2012·Clinical Proteomics·Ghantasala S Sameer KumarAkhilesh Pandey
Jan 8, 2014·Clinical Proteomics·Lavanya BalakrishnanAkhilesh Pandey
Feb 4, 2014·Clinical Proteomics·Lakshmi Dhevi Nagarajha SelvanThottethodi Subrahmanya Keshava Prasad
Feb 19, 2014·Clinical Proteomics·Lavanya BalakrishnanAkhilesh Pandey
Mar 31, 2009·FEBS Letters·Jirina MartinkovaHana Kovarova
Oct 9, 2009·Expert Review of Proteomics·Xin-Qing PengWei-Ming Zhang
Aug 14, 2009·Expert Review of Gastroenterology & Hepatology·Mohamed I F ShariffSimon D Taylor-Robinson
Dec 19, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Shabnam MalikS K Jain
Jun 21, 2011·Biochimica Et Biophysica Acta·Jakob AlbrethsenRuth H Angeletti
Oct 31, 2009·Journal of Chromatography. a·Ching-Seng AngEdouard C Nice
Aug 1, 2012·Mass Spectrometry Reviews·Eva Rodríguez-Suárez, Anthony D Whetton
Mar 11, 2015·Cancer Biology & Therapy·Yashwanth SubbannayyaAditi Chatterjee
Oct 21, 2010·Proteomics·Hexin ChenAkhilesh Pandey
Nov 25, 2011·Proteomics·Mustafa A BarbhuiyaAkhilesh Pandey
Dec 8, 2010·Proteomics. Clinical Applications·Yi-Jen LiaoYi-Ming Arthur Chen
Nov 4, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lauren N BellNaga Chalasani
Apr 14, 2016·Journal of Proteomics·Xiaohua XingJingfeng Liu
Mar 28, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lishan WangYongyong Shi
Dec 4, 2014·Clinics and Research in Hepatology and Gastroenterology·Wei WeiJing-Feng Liu
May 2, 2015·Omics : a Journal of Integrative Biology·Krishna R MurthyAkhilesh Pandey
Jul 12, 2011·Cancer Biology & Therapy·Harsh PawarAkhilesh Pandey
Mar 31, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chanida Puangpila, Ziad El Rassi
Mar 28, 2013·International Journal of Proteomics·Valerie C WasingerYunki Yau
Oct 16, 2013·Clinical Proteomics·Vinuth N PuttamalleshT S Keshava Prasad
Mar 26, 2015·PloS One·Katharina BertramAgnes Görlach
Mar 24, 2015·PloS One·Dinari A HarrisJustin W Taraska

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