PMID: 7372503Jan 1, 1980Paper

A quantitative study of N-acetyl-beta-glucosaminidase activity in unfixed tissue sections of the guinea-pig thyroid gland

The Histochemical Journal
W R Robertson

Abstract

A post-coupling procedure for the quantitative measurement of N-acetyl-beta-glucosaminidase activity in unfixed tissue sections of guinea-pig thyroid is described. The method depends on the cleaving of a naphthol AS-BI substrate and the insoluble reaction product is post-coupled with Fast Garnet GBC salt (in acetate buffer, pH 6.2) at 4 degrees C. Even though this enzyme is localized in the lysosomes, an inert colloid stabiliser, polyvinyl alcohol (G18/140) is included in the reaction medium to allow the use of the optimal substrate concentration (0.5 mg/ml) whilst employing a low concentration (5%) of ethylene glycol monomethyl ether. The high molecular weight (90 000) grade of polyvinyl alcohol used did not stabilize the lysosomal membrane, although a lower molecular weight (15 000) grade of polyvinyl alcohol (G04/140) may do. The enzyme activity was not affected by the metal ions Ca2+ and Zn2+ and was totally abolished by the specific inhibitor 2-acetamido-2-deoxy-D-gluconolactone.

References

Jun 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A D Shannon
Aug 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J E Scott
Sep 1, 1973·Clinical Science and Molecular Medicine·L BitenskyJ Chayen
Jun 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·M ChvapilC F Zukoski
Nov 1, 1967·The Biochemical Journal·R G Price, N Dance
Apr 1, 1968·The Biochemical Journal·D Robinson, J L Stirling
Mar 1, 1957·The Biochemical Journal·D PUGHP G WALKER
May 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M HAYASHI
Jan 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D PUGH, P G WALKER
Jan 1, 1961·The Biochemical Journal·J BorooahP G Walker

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Citations

May 1, 1989·The Histochemical Journal·B J BarrowT P Pretlow
Jul 1, 1989·The Histochemical Journal·C J Van Noorden, I M Vogels
Mar 1, 1995·Microscopy Research and Technique·T G PretlowT P Pretlow
Aug 1, 1989·Molecular and Cellular Endocrinology·H PerrildW R Robertson

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