A quantitative study of the neuronal nitric oxide synthase expression in the superficial layers of the adult rat superior colliculus after perinatal enucleation

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
Ruben Ernesto de Bittencourt-NavarreteRosalia Mendez-Otero

Abstract

The level of expression of the neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of rat superior colliculus (SC) was investigated in adult rats after neonatal enucleation using two biochemical methods: (1) measurement of the in vitro specific-activity of NOS by the conversion of [3H]-arginine to [3H]-citrulline and (2) immunochemical analysis by western blotting and densitometry of immunoreactive bands using antibodies that recognise the three prominent isoforms of nNOS, alpha, beta and gamma. A total of 20 Lister rats were used in this study. We have shown that the deprivation of the retinocollicular projections at early postnatal ages induces no significant change in the specific-activity of nNOS. We also have shown that the deafferentation does not significantly influence either the total amount of nNOS in the SC superficial layers or the relative contribution ratio of nNOS isoforms. In conclusion, the expression and activity of nNOS in the SC retinorecipient layers was shown not to be dependent on the presence of retinal afferents during development.

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Citations

Oct 24, 2007·Brain Research Reviews·Arthur Giraldi-GuimarãesRosalia Mendez-Otero
Sep 27, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Marucia ChacurLuiz R G Britto
Mar 11, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R E de Bittencourt-NavarreteR Mendez-Otero
Jan 1, 2009·Journal of Allergy·Caleb Kelly, Venu Gangur
Jul 1, 2014·Journal of Epilepsy Research·Chang Yeob LeeHye-Jin Moon

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