A quartz crystal resonant sensor (QCRS) study of HSA--drug interactions

The Analyst
K D PaveyF Paul

Abstract

Human serum albumin (HSA) was immobilised on the gold surface of a quartz crystal resonance sensor (QCRS) and exposed to warfarin and diazepam. Distinct decreases in frequency of differing magnitudes were observed upon exposure of the protein to each of the compounds suggesting strongly that a ligand interaction was occurring. Moreover, as sequential exposure in any order was observed to yield distinct repeatable frequency decreases for the ligands indicated, screening for site specific binding may be possible. Identically immobilised bovine serum albumin (BSA) gave no response to either compound.

Citations

Oct 16, 2003·Current Opinion in Pharmacology·Matthew A Cooper
Nov 19, 2003·Journal of Pharmaceutical and Biomedical Analysis·Zhouping WangXiao Zhang
Jan 25, 2011·Physical Chemistry Chemical Physics : PCCP·Vladimir M Gun'koSergey V Mikhalovsky
Apr 18, 2006·Journal of Pharmaceutical and Biomedical Analysis·Hua ChenZhujun Zhang
Feb 7, 2015·Biosensors & Bioelectronics·David C FerrierPhilip J W Hands
Dec 10, 2008·The Journal of Physical Chemistry. B·Jingjing HuTongwen Xu

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